Activation of Arachidonate Release From CD34+ Cells Compared With Other Cell Types
| Cell Type (n) . | Arachidonate Release Stimulated by . | |||
|---|---|---|---|---|
| DMSO . | DMSO + A23187 . | TPA . | TPA + A23187 . | |
| HL-60 (5) | 1.1 ± 0.1* | 1.2 ± 0.2 | 1.1 ± 0.1 | 1.6 ± 0.2 |
| TF-1 (10) | 3.1 ± 0.2 | 5.2 ± 0.5† | 3.6 ± 0.2 | 17.0 ± 1.2† |
| CD34+ (11) | 4.8 ± 0.6 | 6.5 ± 1.0† | 6.4 ± 0.8‡ | 24.5 ± 4.4‡ |
| Neutrophils (7) | 2.7 ± 0.2 | 7.3 ± 1.0‡ | 10.1 ± 1.11-153 | 18.5 ± 1.11-153 |
| Monocytes (10) | 6.9 ± 0.7 | 17.5 ± 2.41-153 | 17.1 ± 1.81-153 | 26.1 ± 2.21-153 |
| Cell Type (n) . | Arachidonate Release Stimulated by . | |||
|---|---|---|---|---|
| DMSO . | DMSO + A23187 . | TPA . | TPA + A23187 . | |
| HL-60 (5) | 1.1 ± 0.1* | 1.2 ± 0.2 | 1.1 ± 0.1 | 1.6 ± 0.2 |
| TF-1 (10) | 3.1 ± 0.2 | 5.2 ± 0.5† | 3.6 ± 0.2 | 17.0 ± 1.2† |
| CD34+ (11) | 4.8 ± 0.6 | 6.5 ± 1.0† | 6.4 ± 0.8‡ | 24.5 ± 4.4‡ |
| Neutrophils (7) | 2.7 ± 0.2 | 7.3 ± 1.0‡ | 10.1 ± 1.11-153 | 18.5 ± 1.11-153 |
| Monocytes (10) | 6.9 ± 0.7 | 17.5 ± 2.41-153 | 17.1 ± 1.81-153 | 26.1 ± 2.21-153 |
CD34+ cells were mobilized from the bone marrow into the peripheral blood and selected on the basis of their CD34 antigen expression. The mean number of monocytes contaminating the CD34+ cell preparations was 2.1% ± 1.0%. TF-1 and CD34+ cells were incubated for 18 hours without growth factors before testing. Neutrophils and monocytes were tested on the day of purification. All cells were radiolabelled with3H-AA and stimulated with either 500 ng/mL TPA or DMSO diluent for 10 minutes, followed by 1 μmol/L A23187 or PBS control for 20 minutes, and AA release was measured as described in Materials and Methods. AA release was expressed as a percentage of the total cell radioactivity, which was 76,341 ± 14,590 cpm/106 HL-60 cells, 92,049 ± 5268 cpm/106 TF-1 cells, 171,863 ± 39,371 cpm/106 CD34 cells, 69,838 ± 12,788 cpm/106 neutrophils, and 61,505 ± 10,380 cpm/106 monocytes. Values are the percentage of cellular radioactivity. P values show the significance of difference from DMSO controls measured by Student’s paired t-test.
The data shown are the mean ± 1 SE of the number of experiments (n).
.05 > P > .01.
.01 > P > .001.
.001 > P.