Table 1. Generation of 3... | American Society of Hematology

Table 1.

Generation of 3 Leukemia-Reactive CTL Lines

	CTL Line
	1	2	3
No. of positive wells harvested	309	389	525
No. of T cells harvested	0.2 × 10⁹	1.3 × 10⁹	1.7 × 10⁹
Phenotype of CTL line
CD3	97%	97%	99%
CD4	92%	82%	86%
CD8	9%	18%	14%
TCRαβ	97%	96%	99%
TCRγδ	<1%	2%	<1%
Lysis^* of CML-MNC	30	24	18
Lysis^* of PHA-blast patient	0	0	0
Lysis^* of BM stromal cells patient	8	NT	10
Lysis^* of PHA-blast donor	3	0	1

	CTL Line
	1	2	3
No. of positive wells harvested	309	389	525
No. of T cells harvested	0.2 × 10⁹	1.3 × 10⁹	1.7 × 10⁹
Phenotype of CTL line
CD3	97%	97%	99%
CD4	92%	82%	86%
CD8	9%	18%	14%
TCRαβ	97%	96%	99%
TCRγδ	<1%	2%	<1%
Lysis^* of CML-MNC	30	24	18
Lysis^* of PHA-blast patient	0	0	0
Lysis^* of BM stromal cells patient	8	NT	10
Lysis^* of PHA-blast donor	3	0	1

Abbreviation: NT, not tested.

Percentage specific lysis in a ⁵¹Cr-release assay at a 10:1 effector:target ratio.