Heat–Shock-Induced Apoptosis
| Hours . | Temperature . | H9 3D8 . | Clone No. 2 . | Clone No. 10 . | Clone No. 16 . |
|---|---|---|---|---|---|
| 24 | 37 | 2.4 ± 0.5 | 2.1 ± 2.6 | 3.3 ± 2.7 | 2.8 ± 3.2 |
| 24 | 41 | 10.9 ± 1.1 | 16.9 ± 1.2 | 23.1 ± 3.1 | 18.2 ± 2.1 |
| 48 | 37 | 2.4 ± 0.9 | 2.1 ± 3.4 | 3.3 ± 3.3 | 2.8 ± 3.7 |
| 48 | 41 | 15 ± 0.6 | 28 ± 4.2 | 38.7 ± 3.5 | 32.5 ± 3.9 |
| Hours . | Temperature . | H9 3D8 . | Clone No. 2 . | Clone No. 10 . | Clone No. 16 . |
|---|---|---|---|---|---|
| 24 | 37 | 2.4 ± 0.5 | 2.1 ± 2.6 | 3.3 ± 2.7 | 2.8 ± 3.2 |
| 24 | 41 | 10.9 ± 1.1 | 16.9 ± 1.2 | 23.1 ± 3.1 | 18.2 ± 2.1 |
| 48 | 37 | 2.4 ± 0.9 | 2.1 ± 3.4 | 3.3 ± 3.3 | 2.8 ± 3.7 |
| 48 | 41 | 15 ± 0.6 | 28 ± 4.2 | 38.7 ± 3.5 | 32.5 ± 3.9 |
Cells were heated for 4 hours from 37°C to 45°C. Subsequently, the cells were cultured for 24 to 48 hours at 37°C until apoptosis was determined on the single cell level by measuring the DNA content of individual nuclei by flow cytometry. Data represent the mean of triplicates ± SD.