Effect of F Peptide on CXCR4 Expression on Monocytes
| Preincubation (37°C for 60 min) . | Antibody . | Percentage of Stained Cells . | ||
|---|---|---|---|---|
| Medium . | Staurosporine3-150 . | Calphostin C3-150 . | ||
| Medium | (−) | 2.9% | 2.3% | 0.5% |
| Medium | Anti-CXCR4 | 71.4% | 42.2% | 78.0% |
| F peptide | Anti-CXCR4 | 26.4% | 43.9% | 52.8% |
| SDF-1α | Anti-CXCR4 | 3.3% | 0.6% | 0.7% |
| Medium | Mouse IgG | 8.1% | 2.5% | 0.6% |
| Preincubation (37°C for 60 min) . | Antibody . | Percentage of Stained Cells . | ||
|---|---|---|---|---|
| Medium . | Staurosporine3-150 . | Calphostin C3-150 . | ||
| Medium | (−) | 2.9% | 2.3% | 0.5% |
| Medium | Anti-CXCR4 | 71.4% | 42.2% | 78.0% |
| F peptide | Anti-CXCR4 | 26.4% | 43.9% | 52.8% |
| SDF-1α | Anti-CXCR4 | 3.3% | 0.6% | 0.7% |
| Medium | Mouse IgG | 8.1% | 2.5% | 0.6% |
Monocytes were preincubated with F peptide or SDF-1α for 60 minutes at 37°C followed by incubation with anti-CXCR4 antibody or mouse IgG for 30 minutes at 4°C. The cells were then stained with FITC-conjugated antimouse IgG and subjected to FACS analyses.
Monocytes were preincubated with staurosporine or calphostin C before treatment with F peptide or SDF-1α.