Ability of LMMA to Abrogate the Suppressive Activity of GVHR Cells and to Inhibit Nitrite Synthesis
| Normal DBA/2 Spleen Cells Cocultured With* . | Mitogen Stimulation . | |||||
|---|---|---|---|---|---|---|
| Con A . | LPS . | |||||
| [3H]Thymidine Incorporation . | Nitrite Synthesis2-155 . | IFN-γ2-154 . | [3H]Thymidine Incorporation . | Nitrite Synthesis2-155 . | IFN-γ2-154 . | |
| Normal B10.D2 spleen cells | 37,793 ± 3,605 | 0 | ND2-154 | 28,280 ± 6,000 | 0 | ND2-154 |
| GVHR spleen cells | 563 ± 176 [1.4%]2-153 | 24.3 ± 0.7 | 852 ± 462-154 | 165 ± 119 [0.5]2-153 | 25.3 ± 2.6 | 22.5 ± 52-154 |
| GVHR spleen cells + LMMA† | 16,938 ± 1,923 [45%]2-153 | 13.0 ± 0.8 | ND2-154 | 910 ± 180 [3.2]2-153 | 13.0 ± 2.3 | ND2-154 |
| GVHR spleen cells + α IFN-γ MoAb‡ | 12,538 ± 2,232 [33%]2-153 | 12.3 ± 1.6 | 2 ± 0.92-154 | 2,140 ± 480 [7.5]2-153 | 11.6 ± 2.4 | 02-154 |
| Normal DBA/2 Spleen Cells Cocultured With* . | Mitogen Stimulation . | |||||
|---|---|---|---|---|---|---|
| Con A . | LPS . | |||||
| [3H]Thymidine Incorporation . | Nitrite Synthesis2-155 . | IFN-γ2-154 . | [3H]Thymidine Incorporation . | Nitrite Synthesis2-155 . | IFN-γ2-154 . | |
| Normal B10.D2 spleen cells | 37,793 ± 3,605 | 0 | ND2-154 | 28,280 ± 6,000 | 0 | ND2-154 |
| GVHR spleen cells | 563 ± 176 [1.4%]2-153 | 24.3 ± 0.7 | 852 ± 462-154 | 165 ± 119 [0.5]2-153 | 25.3 ± 2.6 | 22.5 ± 52-154 |
| GVHR spleen cells + LMMA† | 16,938 ± 1,923 [45%]2-153 | 13.0 ± 0.8 | ND2-154 | 910 ± 180 [3.2]2-153 | 13.0 ± 2.3 | ND2-154 |
| GVHR spleen cells + α IFN-γ MoAb‡ | 12,538 ± 2,232 [33%]2-153 | 12.3 ± 1.6 | 2 ± 0.92-154 | 2,140 ± 480 [7.5]2-153 | 11.6 ± 2.4 | 02-154 |
Abbreviation: ND, not determined.
GVHR spleen cells at day 9 after grafting were tested for their suppressive activity on the mitogenic response of normal DBA/2 spleen cells to Con A and LPS and of nitrite synthesis. This experiment is representative of 4 other experiments.
The cell populations added to the culture were used at the final concentration of 106/mL. LMMA diluted in PBS was added at the beginning of the culture and used at a 1.5 mmol/L final concentration.
The concentration of anti–IFN-γ MoAb was 1.5 μg/mL. MoAb was added at the beginning of the culture. Normal hamster IgG (1.75 μg/mL) had no effect on the suppression (results not shown).
Numbers in square brackets represent the percentage of the control group (normal DBA/2 cells cocultured with normal B10.D2 cells).
Concentration (μmol/L) in the supernatant.
Concentration in pg/mL.