Effect of Ca2+ Ions, Phospholipids, and Bovine Factor Va on Trocarin- and Factor Xa-Mediated Prothrombin Activation
| Activator . | v (pmol prothrombin/ min/μg) . | Relative Rate . |
|---|---|---|
| Trocarin | 0.81 | 1 |
| Trocarin, Ca2+ | 93 | 115 |
| Trocarin, Ca2+, PL | 3,617 | 4,465 |
| Trocarin, Ca2+, Va | 2,091 | 2,581 |
| Trocarin, Ca2+, PL, Va | 12,809 | 15,814 |
| Bovine Xa | 0.19 | 1 |
| Bovine Xa, Ca2+ | 241 | 1,268 |
| Bovine Xa, Ca2+, PL | 9,687 | 50,984 |
| Bovine Xa, Ca2+, Va | 2,757 | 14,511 |
| Bovine Xa, Ca2+, PL, Va | 26,155 | 137,658 |
| Human Xa | 0.08 | 1 |
| Human Xa, Ca2+ | 205 | 2,563 |
| Human Xa, Ca2+, PL | 20,407 | 255,088 |
| Human Xa, Ca2+, Va | 4,668 | 58,350 |
| Human Xa, Ca2+, PL, Va | 49,132 | 614,150 |
| Activator . | v (pmol prothrombin/ min/μg) . | Relative Rate . |
|---|---|---|
| Trocarin | 0.81 | 1 |
| Trocarin, Ca2+ | 93 | 115 |
| Trocarin, Ca2+, PL | 3,617 | 4,465 |
| Trocarin, Ca2+, Va | 2,091 | 2,581 |
| Trocarin, Ca2+, PL, Va | 12,809 | 15,814 |
| Bovine Xa | 0.19 | 1 |
| Bovine Xa, Ca2+ | 241 | 1,268 |
| Bovine Xa, Ca2+, PL | 9,687 | 50,984 |
| Bovine Xa, Ca2+, Va | 2,757 | 14,511 |
| Bovine Xa, Ca2+, PL, Va | 26,155 | 137,658 |
| Human Xa | 0.08 | 1 |
| Human Xa, Ca2+ | 205 | 2,563 |
| Human Xa, Ca2+, PL | 20,407 | 255,088 |
| Human Xa, Ca2+, Va | 4,668 | 58,350 |
| Human Xa, Ca2+, PL, Va | 49,132 | 614,150 |