Superoxide Generation by Murine Bone Marrow Neutrophils
| Group . | NBT+ (%) . | Vmax* . | Cumulative O2− * . | Adjusted Vmax† . | Adjusted Cumulative O2− † . | N . |
|---|---|---|---|---|---|---|
| WT | >95% | 35 ± 8 | 536 ± 83 | 35 ± 8 | 536 ± 8 | 8 |
| X-CGD | 0 | 0 | 0 | 0 | 0 | 4 |
| X-CGD + MSCVm91Neo | ||||||
| 11 | 54% | 2.6 | 32 | 4.8 | 59 | 1 |
| 12 | 68% | 6.1 | 128 | 9 | 188 | 1 |
| 26 | 59% | 2.2 | 40 | 3.7 | 68 | 1 |
| 27 | 70% | 8.1 | 160 | 11.6 | 228 | 1 |
| Mean | 5 ± 3 | 90 ± 64 | 7 ± 4 | 136 ± 85 | 4 |
| Group . | NBT+ (%) . | Vmax* . | Cumulative O2− * . | Adjusted Vmax† . | Adjusted Cumulative O2− † . | N . |
|---|---|---|---|---|---|---|
| WT | >95% | 35 ± 8 | 536 ± 83 | 35 ± 8 | 536 ± 8 | 8 |
| X-CGD | 0 | 0 | 0 | 0 | 0 | 4 |
| X-CGD + MSCVm91Neo | ||||||
| 11 | 54% | 2.6 | 32 | 4.8 | 59 | 1 |
| 12 | 68% | 6.1 | 128 | 9 | 188 | 1 |
| 26 | 59% | 2.2 | 40 | 3.7 | 68 | 1 |
| 27 | 70% | 8.1 | 160 | 11.6 | 228 | 1 |
| Mean | 5 ± 3 | 90 ± 64 | 7 ± 4 | 136 ± 85 | 4 |
Superoxide dismutase-inhibitable O2−production was measured in Percoll-purified BM neutrophils (79% ± 9% neutrophils; mean + standard deviation [SD]) using a continuous cytochrome C reduction assay, as described in Methods. Data are the mean ± SD. N indicates the number of mice studied. MSCV-m91Neo–transplant recipients were studied at 11 months (2 mice) or 18 months (2 mice) posttransplant. Also shown are the percentage of NADPH oxidase-positive neutrophils in each preparation, as measured by the NBT test.
Vmax = nm O2−/min/107 cells; cumulative O2− = nm O2−/30 min/107 cells.
Adjusted value if 100% of neutrophils were NBT-positive after MSCV-m91Neo–mediated gene transfer.