Peripheral Blood Neutrophil NADPH Oxidase Activity in X-CGD Mice Transplanted with MSCV-91Neo–Transduced BM
| Time Posttransplant (mo) . | X-CGD + MSCV-m91Neo . | X-CGD + MSCV-h91Neo . | ||||
|---|---|---|---|---|---|---|
| Mean NBT+ (%) . | (Range) . | N . | Mean NBT+ (%) . | (Range) . | N . | |
| 2-3 | 56 | (48-67) | 12 | 4.4 | (1-11) | 5 |
| 5-7 | 49 | (28-73) | 15 | 3.2 | (0-8) | 5 |
| 9-12 | 52 | (13-92) | 15 | 4.6 | (0-11) | 5 |
| 15 | 46 | (9-75) | 7 | — | — | — |
| 18 | 54 | (50-58) | 2 | 1.1* | (0-3) | 5 |
| 24 | — | — | — | 1.2* | (0.5-3) | 3 |
| Time Posttransplant (mo) . | X-CGD + MSCV-m91Neo . | X-CGD + MSCV-h91Neo . | ||||
|---|---|---|---|---|---|---|
| Mean NBT+ (%) . | (Range) . | N . | Mean NBT+ (%) . | (Range) . | N . | |
| 2-3 | 56 | (48-67) | 12 | 4.4 | (1-11) | 5 |
| 5-7 | 49 | (28-73) | 15 | 3.2 | (0-8) | 5 |
| 9-12 | 52 | (13-92) | 15 | 4.6 | (0-11) | 5 |
| 15 | 46 | (9-75) | 7 | — | — | — |
| 18 | 54 | (50-58) | 2 | 1.1* | (0-3) | 5 |
| 24 | — | — | — | 1.2* | (0.5-3) | 3 |
The percentage of NBT-positive neutrophils, an assay for NADPH oxidase activity, was determined as described in Methods. Nindicates the number of mice studied from each group at each timepoint. NBT-positive neutrophils were never detected in untransplanted X-CGD mice.
Mean includes data from both blood and bone marrow neutrophil samples.