Table 1.

Appearance of Tryptase+ Cells and Chymase+ Cells in Culture With CD34+ Cord Blood Cells and SCF

Weeks in Culture Tryptase+ Cells (%)Chymase+ Cells (%)
1   7 ± 2  0  
78 ± 4  0  
3  96 ± 3  0  
4  100  
7  100  0  
10  100   2 ± 1  
14 100   8 ± 1  
22  100  40 ± 3 
32  100  91 ± 4  
36  100  100  
40 100  100  
48  100  100  
60  100  100 
Weeks in Culture Tryptase+ Cells (%)Chymase+ Cells (%)
1   7 ± 2  0  
78 ± 4  0  
3  96 ± 3  0  
4  100  
7  100  0  
10  100   2 ± 1  
14 100   8 ± 1  
22  100  40 ± 3 
32  100  91 ± 4  
36  100  100  
40 100  100  
48  100  100  
60  100  100 

The cultured cells shown in Fig 2A were processed for immunocytochemical staining with an MoAb against tryptase or chymase. The results shown are from one representative experiment of three. Similar results were obtained in the other two experiments. Values are expressed as the mean ± SD.

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