Appearance of Tryptase+ Cells and Chymase+ Cells in Culture With CD34+ Cord Blood Cells and SCF
Weeks in Culture . | Tryptase+ Cells (%) . | Chymase+ Cells (%) . |
---|---|---|
1 | 7 ± 2 | 0 |
2 | 78 ± 4 | 0 |
3 | 96 ± 3 | 0 |
4 | 100 | 0 |
7 | 100 | 0 |
10 | 100 | 2 ± 1 |
14 | 100 | 8 ± 1 |
22 | 100 | 40 ± 3 |
32 | 100 | 91 ± 4 |
36 | 100 | 100 |
40 | 100 | 100 |
48 | 100 | 100 |
60 | 100 | 100 |
Weeks in Culture . | Tryptase+ Cells (%) . | Chymase+ Cells (%) . |
---|---|---|
1 | 7 ± 2 | 0 |
2 | 78 ± 4 | 0 |
3 | 96 ± 3 | 0 |
4 | 100 | 0 |
7 | 100 | 0 |
10 | 100 | 2 ± 1 |
14 | 100 | 8 ± 1 |
22 | 100 | 40 ± 3 |
32 | 100 | 91 ± 4 |
36 | 100 | 100 |
40 | 100 | 100 |
48 | 100 | 100 |
60 | 100 | 100 |
The cultured cells shown in Fig 2A were processed for immunocytochemical staining with an MoAb against tryptase or chymase. The results shown are from one representative experiment of three. Similar results were obtained in the other two experiments. Values are expressed as the mean ± SD.