Binding of b2a2 17mer Peptide Analogs to HLA DR2a
| aa Sequence . | Relative Binding Capacity IC50(μmol/L) 3-150 . |
|---|---|
| IPLTINKEEALQRPVAS | 5 |
| APLTINKEEALQRPVAS | 7 |
| IALTINKEEALQRPVAS | 9 |
| IPATINKEEALQRPVAS | 8.6 |
| IPLAINKEEALQRPVAS | 4.7 |
| IPLTANKEEALQRPVAS | >70 |
| IPLTIAKEEALQRPVAS | 4 |
| IPLTINAEEALQRPVAS | 4 |
| IPLTINKAEALQRPVAS | 1.2 |
| IPLTINKEAALQRPVAS | 2.7 |
| IPLTINKEEAAQRPVAS | 9 |
| IPLTINKEEALARPVAS | 7 |
| IPLTINKEEALQAPVAS | >70 |
| IPLTINKEEALQRAVAS | 12 |
| IPLTINKEEALQRPAAS | 12 |
| IPLTINKEEALQRPVAA | 15 |
| aa Sequence . | Relative Binding Capacity IC50(μmol/L) 3-150 . |
|---|---|
| IPLTINKEEALQRPVAS | 5 |
| APLTINKEEALQRPVAS | 7 |
| IALTINKEEALQRPVAS | 9 |
| IPATINKEEALQRPVAS | 8.6 |
| IPLAINKEEALQRPVAS | 4.7 |
| IPLTANKEEALQRPVAS | >70 |
| IPLTIAKEEALQRPVAS | 4 |
| IPLTINAEEALQRPVAS | 4 |
| IPLTINKAEALQRPVAS | 1.2 |
| IPLTINKEAALQRPVAS | 2.7 |
| IPLTINKEEAAQRPVAS | 9 |
| IPLTINKEEALARPVAS | 7 |
| IPLTINKEEALQAPVAS | >70 |
| IPLTINKEEALQRAVAS | 12 |
| IPLTINKEEALQRPAAS | 12 |
| IPLTINKEEALQRPVAA | 15 |
IC50 denotes the concentration of b2a2 peptide or an analog thereof required to inhibit the binding of fluorescent labeled CLIP peptide p87-10136 (VSKMRMATPLLMQAL) by 50%. IC50 of unlabeled CLIP peptide was 0.2 μmol/L.