Frequencies of Lin− Populations in Bone Marrow
Sorted Population* . | Frequency (% of BM MNC) . | Frequency of TdT+ Cells (% of BM MNC) . | TdT+ Cells in Sorted Population (%) . | Fold Enrichment for Lin−TdT+ Cells . |
---|---|---|---|---|
Lin− c-kitHi | 1.40 ± .09 | 0.080 | 5.7 ± 1.3 | 19 |
Lin− c-kitLo | 0.56 ± .09 | 0.200 | 35.4 ± 5.1 | 116 |
Lin− c-kit− | 0.33 ± .03 | 0.024 | 7.4 ± 1.2 | 24 |
Sorted Population* . | Frequency (% of BM MNC) . | Frequency of TdT+ Cells (% of BM MNC) . | TdT+ Cells in Sorted Population (%) . | Fold Enrichment for Lin−TdT+ Cells . |
---|---|---|---|---|
Lin− c-kitHi | 1.40 ± .09 | 0.080 | 5.7 ± 1.3 | 19 |
Lin− c-kitLo | 0.56 ± .09 | 0.200 | 35.4 ± 5.1 | 116 |
Lin− c-kit− | 0.33 ± .03 | 0.024 | 7.4 ± 1.2 | 24 |
Lin− cells were stained, gated, and sorted as shown in Fig 1. Bone marrow frequencies of Lin−c-kit+, Lin− c-kitLo, and Lin− c-kit− cells reflect means from three or four independent experiments with marrow pooled from 9 or 10 mice. Frequencies of TdT+ populations within bone marrow were calculated from percentages of TdT+ cells within the indicated subsets. Fold enrichment of Lin−TdT+ cells within each subset is relative to the frequency among bone marrow mononuclear cells (0.304%).