Table 1. Synthetic Oligonucleotide... | American Society of Hematology

Table 1.

Synthetic Oligonucleotide Primers Used to Amplify Genomic, PAC, and Plasmid DNA by PCR

Primer ID	Sequence^*	Position
EPCR-3	ACCTAACGCACGTGCTGG	2634 to 2651^†
EPCR-4	AGGTCCGCTCCTGGTGC^‡	2791 to 2776^†
EPCR-5	GGAGCTCCTTTGTGAGTT	4103 to 4120^†
EPCR-8	AATTCCCGCAGTTCATAC^‡	4235 to 4218^†
EP1A	AGACGGTCCTCACTTCTC	−94 to −77^†
EP1B	GATAGACTGAGATTCTCC^‡	118 to 99^†
EP2C	GAGTTACTGTCAGCGTCA	2383 to 2400^†
EP2B	CCCAGCAATCTTCAAAGG^‡	2957 to 2940^†
EP3A	ACACCTGGCACCCTCTCT	3164 to 3981^†
EP3B	CATCCTTCAGGTCCATCC^‡	4370 to 4353^†
EP4A	GACTCAAATCATGGACTC	4484 to 4501^†
EP4B	CCCAACTCCATTATCTGT^‡	4948 to 4931^†
RACE-2	AGCAGCGGATGGTCAGAGGA	367 to 348^1-153
RACE-9	GTTGCCCTGGTACCACACGT	165 to 147^1-153
RACE-11	TGAGGCGTCTTGGCTACA	90 to 73^1-153

Primer ID	Sequence^*	Position
EPCR-3	ACCTAACGCACGTGCTGG	2634 to 2651^†
EPCR-4	AGGTCCGCTCCTGGTGC^‡	2791 to 2776^†
EPCR-5	GGAGCTCCTTTGTGAGTT	4103 to 4120^†
EPCR-8	AATTCCCGCAGTTCATAC^‡	4235 to 4218^†
EP1A	AGACGGTCCTCACTTCTC	−94 to −77^†
EP1B	GATAGACTGAGATTCTCC^‡	118 to 99^†
EP2C	GAGTTACTGTCAGCGTCA	2383 to 2400^†
EP2B	CCCAGCAATCTTCAAAGG^‡	2957 to 2940^†
EP3A	ACACCTGGCACCCTCTCT	3164 to 3981^†
EP3B	CATCCTTCAGGTCCATCC^‡	4370 to 4353^†
EP4A	GACTCAAATCATGGACTC	4484 to 4501^†
EP4B	CCCAACTCCATTATCTGT^‡	4948 to 4931^†
RACE-2	AGCAGCGGATGGTCAGAGGA	367 to 348^1-153
RACE-9	GTTGCCCTGGTACCACACGT	165 to 147^1-153
RACE-11	TGAGGCGTCTTGGCTACA	90 to 73^1-153

The sequence of all oligonucleotide primers is given in the 5′ to 3′ direction.

Numbering according to Fig 2.

Antisense primer.

Numbering according to cDNA position.8