IL-6R (gp80) Expression and Clonogenic Growth in OPM-2 Parental Line and Transfectant Clones With or Without ATRA Pretreatment
| Cell Line . | A. IL-6Rα (mean channel no.) . | B. Clonogenic Growth (colony/well, n = 3) . | |||
|---|---|---|---|---|---|
| (a) Mean Channel No. . | (b) Post/Pre-ATRA* (%) . | Control . | +ATRA† . | % Inhibition . | |
| OPM-2 parental line | 217 (100%)‡ | 130/225 (58) | 186 ± 55 | 33 ± 8 | 82 |
| Transfectants, pooled | 619 (267) | — | — | — | — |
| Clone A1 | 164 (76) | 108/267 (40) | 155 ± 54 | 13 ± 10 | 92 |
| Clone C2 | 436 (201) | 714/324 (220) | 95 ± 28 | 4 ± 1 | 96 |
| Clone C3 | 315 (145) | 457/341 (134) | 125 ± 80 | 5 ± 3 | 96 |
| Clone C4 | 1,043 (480) | 853/636 (134) | 178 ± 62 | 4 ± 5 | 98 |
| Clone C5 | 1,282 (590) | 943/677 (139) | 355 ± 48 | 13 ± 11 | 96 |
| Clone D6 | 709 (326) | 1,190/700 (170) | 119 ± 21 | 11 ± 11 | 91 |
| Cell Line . | A. IL-6Rα (mean channel no.) . | B. Clonogenic Growth (colony/well, n = 3) . | |||
|---|---|---|---|---|---|
| (a) Mean Channel No. . | (b) Post/Pre-ATRA* (%) . | Control . | +ATRA† . | % Inhibition . | |
| OPM-2 parental line | 217 (100%)‡ | 130/225 (58) | 186 ± 55 | 33 ± 8 | 82 |
| Transfectants, pooled | 619 (267) | — | — | — | — |
| Clone A1 | 164 (76) | 108/267 (40) | 155 ± 54 | 13 ± 10 | 92 |
| Clone C2 | 436 (201) | 714/324 (220) | 95 ± 28 | 4 ± 1 | 96 |
| Clone C3 | 315 (145) | 457/341 (134) | 125 ± 80 | 5 ± 3 | 96 |
| Clone C4 | 1,043 (480) | 853/636 (134) | 178 ± 62 | 4 ± 5 | 98 |
| Clone C5 | 1,282 (590) | 943/677 (139) | 355 ± 48 | 13 ± 11 | 96 |
| Clone D6 | 709 (326) | 1,190/700 (170) | 119 ± 21 | 11 ± 11 | 91 |