Table 2.

Comparison of K14 Antigen Expression on Wild-Type, Kpa, and Kpc Transfectants

Kell cDNA Constructs Median of Relative Fluorescence Intensity
Anti-K14
Mock (n = 6) 4 (3-4)  
pRc/CMV-wt (n = 6)  1,519 (895-2,283) 
pRc/CMV-Kpa (n = 6)  445 (335-621) 
pRc/CMV-Kpc (n = 3)  1,612 (1,071-2,025) 
Kell cDNA Constructs Median of Relative Fluorescence Intensity
Anti-K14
Mock (n = 6) 4 (3-4)  
pRc/CMV-wt (n = 6)  1,519 (895-2,283) 
pRc/CMV-Kpa (n = 6)  445 (335-621) 
pRc/CMV-Kpc (n = 3)  1,612 (1,071-2,025) 

The averaged values of relative fluorescence intensity from several experiments (n) are shown with the range of the values indicated in parentheses. The values of relative fluorescence intensity for each cell population transfected with the plasmid constructs are given after normalization for transfection efficiency.

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