Importance of Antigen-Specific Versus Bystander Proliferation in Donor T-Cell Expansion
| . | Donor Cell Origin . | |
|---|---|---|
| Ly5.1+Thy1.2+ (host-reactive +) . | Ly5.2+Thy1.1+ (host-reactive −) . | |
| Day 8 | ||
| CD4 | 28% | 0.8% |
| CD8 | 71% | 0.2% |
| Total | 99% | 1% |
| Day 12 | ||
| CD4 | 33% | 0.6% |
| CD8 | 66% | 0.4% |
| Total | 99% | 1% |
| . | Donor Cell Origin . | |
|---|---|---|
| Ly5.1+Thy1.2+ (host-reactive +) . | Ly5.2+Thy1.1+ (host-reactive −) . | |
| Day 8 | ||
| CD4 | 28% | 0.8% |
| CD8 | 71% | 0.2% |
| Total | 99% | 1% |
| Day 12 | ||
| CD4 | 33% | 0.6% |
| CD8 | 66% | 0.4% |
| Total | 99% | 1% |
B6AF1 recipients (Ly5.2+Thy1.2+) received a cell suspension containing 4 × 107 B6.SJL T cells (containing host-reactive T cells) (Ly5.1+Thy1.2+) and 4 × 107Ly5.2+Thy1.1+ non–host-reactive T cells. Results show the mean (N = 3) proportion of donor-derived T cells that were Ly5.2+Thy1.1+ (non–host-reactive) versus Ly5.1+Thy1.2+. Non–host-reactive T cells were harvested from day-60 lethally irradiated B6AF1 recipients transplanted with 107 T-cell–depleted bone marrow cells from B6.PL donors. Trace amounts of B6AF1-derived T cells that could have contaminated the inoculum of non–host-reactive cells were not enumerated by the Ly5.2+Thy1.1+ phenotype.