The Characteristics of Microsatellite Markers for LOH and MSI Analysis
| Name* . | Chromosomal Location . | Repeat Motif . | Primer Sequence 5′-3′ . | Size (bp) . |
|---|---|---|---|---|
| D2S367 | 2p21-22 | (CA)n | Supplied by Perkin Elmer | 300-330 |
| D2S288 | 2p21-22 | (CA)n | AGGGCCTTGCTCTGGATT | 266-298 |
| GGCCAGTGATTGTTCCCC | ||||
| D2S391 | 2p21-22 | (CA)n | Supplied by Perkin Elmer | 125-155 |
| D2S2294 | 2p21-22 | (CA)n | GGCCCATGACTGTAATGC | 211-243 |
| CTGAATGGACACACTGGTTAAG | ||||
| TNF | 6p21.3 | (GT)n | GCCTCTAGATTTCATCCAGCCACA | 95-123 |
| CCTCTCTCCCCTGCAACACACA | ||||
| D6S264 | 6q26 | (CA)n | AGCTGACTTTATGCTGTTCCT | 102-130 |
| TTTTCCATGCCCTTCTATCA | ||||
| APC | 5q | (CA)n | CCCAATTGTATAGATTTAGAAGTC | 155-192 |
| ATCAGAGTATCAGAATTTCT | ||||
| Rb1 | 13q14 | CTTT(T) 4-5 bp repeat | CTCCTCCCTACTTACTTGT | 255-310 |
| AATTAACAAGGTGTGGTGGTACAC | ||||
| P53 | 17p | (CA)n | ACTGCCACTCCTTGCCCCATTC | 105-135 |
| AGGGATACTATTCAGCCCGAGGTG | ||||
| D7S636 | 7q36 | (CA)n | GGAGTGACTGGGCAGGAA | 115-170 |
| AGCTTGTGTGGGGTTTCA | ||||
| LPL | 8p22 | (CA)n(GT)n | CAGTGGGTTATTTGTGGGATA | 105-140 |
| TAGAGCACACTATCCAGGTGA | ||||
| DCC | 18q21 | (TA)n | TCCCTCTAGAAATTGTGTG | 106-160 |
| TGACTTTATCTCATTGGAG | ||||
| D5S107 | 5q12 | (CA)9AA(CA)19(GT)7 | GATCCACTTTAACCCAAATAC | 115-155 |
| GGCATCAACTTGAACAGCAT | ||||
| NF1 | 17q | (CA)n | CAGAGCAAGACCCTGTCT | 170-190 |
| CTCCTAACATTTATTAACCTTA | ||||
| WT1 | 11p3 | (CA)n | AATGAGACTTACTGGGTGAGG | 120-155 |
| TTACACAGTAATTTCAAGCAACGG |
| Name* . | Chromosomal Location . | Repeat Motif . | Primer Sequence 5′-3′ . | Size (bp) . |
|---|---|---|---|---|
| D2S367 | 2p21-22 | (CA)n | Supplied by Perkin Elmer | 300-330 |
| D2S288 | 2p21-22 | (CA)n | AGGGCCTTGCTCTGGATT | 266-298 |
| GGCCAGTGATTGTTCCCC | ||||
| D2S391 | 2p21-22 | (CA)n | Supplied by Perkin Elmer | 125-155 |
| D2S2294 | 2p21-22 | (CA)n | GGCCCATGACTGTAATGC | 211-243 |
| CTGAATGGACACACTGGTTAAG | ||||
| TNF | 6p21.3 | (GT)n | GCCTCTAGATTTCATCCAGCCACA | 95-123 |
| CCTCTCTCCCCTGCAACACACA | ||||
| D6S264 | 6q26 | (CA)n | AGCTGACTTTATGCTGTTCCT | 102-130 |
| TTTTCCATGCCCTTCTATCA | ||||
| APC | 5q | (CA)n | CCCAATTGTATAGATTTAGAAGTC | 155-192 |
| ATCAGAGTATCAGAATTTCT | ||||
| Rb1 | 13q14 | CTTT(T) 4-5 bp repeat | CTCCTCCCTACTTACTTGT | 255-310 |
| AATTAACAAGGTGTGGTGGTACAC | ||||
| P53 | 17p | (CA)n | ACTGCCACTCCTTGCCCCATTC | 105-135 |
| AGGGATACTATTCAGCCCGAGGTG | ||||
| D7S636 | 7q36 | (CA)n | GGAGTGACTGGGCAGGAA | 115-170 |
| AGCTTGTGTGGGGTTTCA | ||||
| LPL | 8p22 | (CA)n(GT)n | CAGTGGGTTATTTGTGGGATA | 105-140 |
| TAGAGCACACTATCCAGGTGA | ||||
| DCC | 18q21 | (TA)n | TCCCTCTAGAAATTGTGTG | 106-160 |
| TGACTTTATCTCATTGGAG | ||||
| D5S107 | 5q12 | (CA)9AA(CA)19(GT)7 | GATCCACTTTAACCCAAATAC | 115-155 |
| GGCATCAACTTGAACAGCAT | ||||
| NF1 | 17q | (CA)n | CAGAGCAAGACCCTGTCT | 170-190 |
| CTCCTAACATTTATTAACCTTA | ||||
| WT1 | 11p3 | (CA)n | AATGAGACTTACTGGGTGAGG | 120-155 |
| TTACACAGTAATTTCAAGCAACGG |
D2S367, D2S288, D2S391, and D2S2944 for LOH analysis; others for MSI analysis.