The Primers for PCR, RT-PCR, and Sequencing
| Primer Name . | Primer Sequences 5′ → 3′ . | Size (bp) . |
|---|---|---|
| RT-PCR of MSH2 (exon 13 plus parts of exon 12 and 14) | ||
| Sense | ATC CAG GCA TGC TTG TGT TG | 547 |
| Antisense | CTT CAC CTG ATA AAG CAT AG | |
| GAPDH | ||
| Sense | CCA CCC ATG GCA AAT TCC ATG GCA | 600 |
| Antisense | CCA CCT GGACTG GAC GGC AGA TCT | |
| RT-PCR of p53 (full-length) | ||
| Fragment 1 (exons 2, 3, and 4 plus parts of exon 1 and 5) | GAC ACG CTT CCC TGG ATT GGC GCA AAA CAT CTT GTT GAG GGC A | 454 |
| Fragment 2 (exons 5 and 6 plus parts of exons 4 and 7) | GTT TCC GTC TGG GCT TCT TGC A GGT ACA GTC AGA GCC AAC CTC | 368 |
| Fragment 3 (exons 6, 7, 8, and 9 plus parts of exons 5 and 10) | TGG CCC CTC CTC AGC ATC TTA CAA GGC CTC ATT CAG CTC TC | 482 |
| Fragment 4 (exons 9, 10, and 11 plus a part of exon 8) | CGG CGC ACA GAG GAA GAG AAT C CGC ACA CCT ATT GCA AGC AAG GG | 444 |
| PCR of MSH2 (exon 13) | ||
| Sense | AGAAAGAAGTTTAAAATCTTGC | 282 |
| Antisense | CTATCTTCAAGGGACTAGG |
| Primer Name . | Primer Sequences 5′ → 3′ . | Size (bp) . |
|---|---|---|
| RT-PCR of MSH2 (exon 13 plus parts of exon 12 and 14) | ||
| Sense | ATC CAG GCA TGC TTG TGT TG | 547 |
| Antisense | CTT CAC CTG ATA AAG CAT AG | |
| GAPDH | ||
| Sense | CCA CCC ATG GCA AAT TCC ATG GCA | 600 |
| Antisense | CCA CCT GGACTG GAC GGC AGA TCT | |
| RT-PCR of p53 (full-length) | ||
| Fragment 1 (exons 2, 3, and 4 plus parts of exon 1 and 5) | GAC ACG CTT CCC TGG ATT GGC GCA AAA CAT CTT GTT GAG GGC A | 454 |
| Fragment 2 (exons 5 and 6 plus parts of exons 4 and 7) | GTT TCC GTC TGG GCT TCT TGC A GGT ACA GTC AGA GCC AAC CTC | 368 |
| Fragment 3 (exons 6, 7, 8, and 9 plus parts of exons 5 and 10) | TGG CCC CTC CTC AGC ATC TTA CAA GGC CTC ATT CAG CTC TC | 482 |
| Fragment 4 (exons 9, 10, and 11 plus a part of exon 8) | CGG CGC ACA GAG GAA GAG AAT C CGC ACA CCT ATT GCA AGC AAG GG | 444 |
| PCR of MSH2 (exon 13) | ||
| Sense | AGAAAGAAGTTTAAAATCTTGC | 282 |
| Antisense | CTATCTTCAAGGGACTAGG |