The Effect of the Late (Day 7) Addition of ATRA on the Granulocytic Differentiation of Cultured Hematopoietic Precursors
| Stage of Development . | Percentage of Cells in Field Showing Morphology . | |||
|---|---|---|---|---|
| Experiment No. 1 . | Experiment No. 2 . | |||
| No ATRA . | ATRA . | No ATRA . | ATRA . | |
| Promyelocytes/blasts | 50 | 20 | 44 | 26 |
| Myelocyte/metamyelocyte | 14 | 23 | 15 | 9 |
| Bands | 30 | 46 | 37 | 63 |
| Segmented nuclei | 3 | 6 | 3 | 1 |
| Monocyte/macrophage | 3 | 5 | 1 | 1 |
| Stage of Development . | Percentage of Cells in Field Showing Morphology . | |||
|---|---|---|---|---|
| Experiment No. 1 . | Experiment No. 2 . | |||
| No ATRA . | ATRA . | No ATRA . | ATRA . | |
| Promyelocytes/blasts | 50 | 20 | 44 | 26 |
| Myelocyte/metamyelocyte | 14 | 23 | 15 | 9 |
| Bands | 30 | 46 | 37 | 63 |
| Segmented nuclei | 3 | 6 | 3 | 1 |
| Monocyte/macrophage | 3 | 5 | 1 | 1 |
Differential counts of cytospins of cells obtained 8 days after initiation of liquid suspension culture of 1,000 initial lin−c-kit+Sca-1+hematopoietic precursors grown in the absence of ATRA (No ATRA) or to which ATRA (final concentration, 1 μmol/L) was added at day 7 (ATRA) after culture initiation. At least 400 cells in each cytospin preparation were counted in one field.