Effect of Peptides on PE Binding to Immobilized Proteins and Cells
| Peptides . | Concentration (μg/mL) . | PE Binding (% of control) . | ||||
|---|---|---|---|---|---|---|
| Targets . | ||||||
| CSA-BSA . | CD36 . | CD36-CHO Cell . | TSP . | C32 Cell . | ||
| None | — | 100 | 100 | 100 | 100 | 100 |
| LF peptide | 10 | 76.1 ± 11.3 | ND | ND | 75.2 ± 13.5 | ND |
| 20 | 42.6 ± 1.2 | ND | ND | 68.0 ± 8.7 | ND | |
| 50 | 41.2 ± 20.9 | ND | 77.3 ± 10.7 | 30.8 ± 2.6 | 68.1 ± 28.9 | |
| 100 | 38.0 ± 14.4 | 85.8 ± 32.7 | 49.0 ± 5.0 | 38.2 ± 4.4 | 39.5 ± 14.6 | |
| 200 | ND | 79.6 ± 26.4 | ND | 20.1 ± 6.7 | ND | |
| 500 | 20.7 ± 1.9 | 58.0 ± 5.6 | 47.8 ± 8.1 | ND | 43.7 ± 8.8 | |
| 1,000 | 21.9 ± 10.1 | 34.9 ± 20.3 | 50.6 ± 10.4 | ND | 37.2 ± 10.9 | |
| FASP peptide | 1,000 | 93.2 ± 16.9 | 107.6 ± 13.3 | 107.6 ± 30.0 | 98.4 ± 2.3 | 93.7 ± 12.4 |
| KPSE peptide | 1,000 | 90.0 ± 18.7 | 106.6 ± 10.1 | 98.2 ± 14.7 | 95.5 ± 10.7 | 108.3 ± 27.4 |
| Peptides . | Concentration (μg/mL) . | PE Binding (% of control) . | ||||
|---|---|---|---|---|---|---|
| Targets . | ||||||
| CSA-BSA . | CD36 . | CD36-CHO Cell . | TSP . | C32 Cell . | ||
| None | — | 100 | 100 | 100 | 100 | 100 |
| LF peptide | 10 | 76.1 ± 11.3 | ND | ND | 75.2 ± 13.5 | ND |
| 20 | 42.6 ± 1.2 | ND | ND | 68.0 ± 8.7 | ND | |
| 50 | 41.2 ± 20.9 | ND | 77.3 ± 10.7 | 30.8 ± 2.6 | 68.1 ± 28.9 | |
| 100 | 38.0 ± 14.4 | 85.8 ± 32.7 | 49.0 ± 5.0 | 38.2 ± 4.4 | 39.5 ± 14.6 | |
| 200 | ND | 79.6 ± 26.4 | ND | 20.1 ± 6.7 | ND | |
| 500 | 20.7 ± 1.9 | 58.0 ± 5.6 | 47.8 ± 8.1 | ND | 43.7 ± 8.8 | |
| 1,000 | 21.9 ± 10.1 | 34.9 ± 20.3 | 50.6 ± 10.4 | ND | 37.2 ± 10.9 | |
| FASP peptide | 1,000 | 93.2 ± 16.9 | 107.6 ± 13.3 | 107.6 ± 30.0 | 98.4 ± 2.3 | 93.7 ± 12.4 |
| KPSE peptide | 1,000 | 90.0 ± 18.7 | 106.6 ± 10.1 | 98.2 ± 14.7 | 95.5 ± 10.7 | 108.3 ± 27.4 |
Spots of immobilized proteins and cells were pretreated with the indicated concentrations of peptide solutions, and the pretreatment solutions were removed before cytoadherence assay. Cytoadherence assays were conducted as described under Materials and Methods and in the legends of Figs 1, 2, 5, and 7. Each value represents the mean ± SD of triplicate determinations.
Abbreviation: ND, not determined.