Table 6. DNA Sequence Analysis of... | American Society of Hematology

Table 6.

DNA Sequence Analysis of TT-Specific T-Cell Clones Derived From MHC Class II–Deficiency Allo-BMT Recipients and Their Donors

No. of Clones		TCRBV		N1-D-N2	TCRBJ
Donor 1	1	1S1	$\underset{̲}{C}$ ASSV	YPRPP	QF $\underset{̲}{FG}$	2S1
	1	2S1	$\underset{̲}{C}$ SA	TKVGLGF	TDTQY $\underset{̲}{FG}$	2S3
	1	5S1	$\underset{̲}{C}$ ASSL	VEG	NTEAF $\underset{̲}{FG}$	1S1
	1	5S1	$\underset{̲}{C}$ ASSL	RAFFVD	EQF $\underset{̲}{FG}$	2S1
	4	5S2	$\underset{̲}{C}$ ASS	YGV	SGANVLT $\underset{̲}{FG}$	2S6
	1	5S6	$\underset{̲}{C}$ ASS	QGLAGV	TDTQY $\underset{̲}{FG}$	2S3
	1	5S6	$\underset{̲}{C}$ ASS	QGLAGI	TDTQY $\underset{̲}{FG}$	2S3
	1	5S6	$\underset{̲}{C}$ ASS	RDSK	NTGELF $\underset{̲}{FG}$	2S2
	1	5S6	$\underset{̲}{C}$ ASS	SGLAVSG	DTQY $\underset{̲}{FG}$	2S3
	1	13S3	$\underset{̲}{C}$ AS	TPDSFMA	T $\underset{̲}{FG}$	1S2
Patient 1	1	2S1	$\underset{̲}{C}$ SAR	GAGART	PLH $\underset{̲}{FG}$	1S6
	1	4S1	$\underset{̲}{C}$ SVE	ERRRR	ETQY $\underset{̲}{FG}$	2S5
	2	4S1	$\underset{̲}{C}$ SV	VSTGAGTAA	EQY $\underset{̲}{FG}$	2S7
	3	5S6	$\underset{̲}{C}$ ASS	QGLAGV	TDTQY $\underset{̲}{FG}$	2S3
	1	10S1	$\underset{̲}{C}$ ASS	PRGGSSLG	TDTQY $\underset{̲}{FG}$	2S3
	1	11S1	$\underset{̲}{C}$ ASS	PMGQIP	EQY $\underset{̲}{FG}$	2S7
	1	13S3	$\underset{̲}{C}$ ASSE	IFVKGADP	TDTQY $\underset{̲}{FG}$	2S3
Donor 2	1	1S1	$\underset{̲}{C}$ ASSV	ATGQ	QETQY $\underset{̲}{FG}$	2S5
	1	2S1	$\underset{̲}{C}$ SA	TGTGKS	EQY $\underset{̲}{FG}$	2S7
	1	5S1	$\underset{̲}{C}$ ASSL	EVH	NYGYT $\underset{̲}{FG}$	1S2
	2	5S1	$\underset{̲}{C}$ ASSL	AGS	SYNEQF $\underset{̲}{FG}$	2S1
	2	5S1	$\underset{̲}{C}$ ASS	SDGQGR	PQH $\underset{̲}{FG}$	2S5
	2	5S1	$\underset{̲}{C}$ AS	GFTAGVS	ETQY $\underset{̲}{FG}$	2S5
	1	5S6	$\underset{̲}{C}$ ASS	SGLAGVS	DTQY $\underset{̲}{FG}$	2S3
	1	6S5	$\underset{̲}{C}$ ASS	FRREP	SYNEQ $\underset{̲}{FG}$	2S1
	1	8S1/2	$\underset{̲}{C}$ ASSL	GRGG	TDTQY $\underset{̲}{FG}$	2S3
	1	8S1/2	$\underset{̲}{C}$ ASSL	GQAI	GYT $\underset{̲}{FG}$	1S2
	3	8S1/2	$\underset{̲}{C}$ ASS	SSGKH	EQF $\underset{̲}{FG}$	2S1
	1	8S1/2	$\underset{̲}{C}$ AS	RWGA	EAF $\underset{̲}{FG}$	1S1
	11	21S3	$\underset{̲}{C}$ ASS	FTRK	NTEAF $\underset{̲}{FG}$	1S1
Patient 2	1	2S1	$\underset{̲}{C}$ SAR	DKNAA	QF $\underset{̲}{FG}$	2S1
	2	4S1	$\underset{̲}{C}$ SV	QRLD	TIY $\underset{̲}{FG}$	1S3
	11	6S1	$\underset{̲}{C}$ ASS	QQLGGA	DTQY $\underset{̲}{FG}$	2S3
	3	6S7a	$\underset{̲}{C}$ ASS	PALAGGP	YEQY $\underset{̲}{FG}$	2S7
	2	13S3	$\underset{̲}{C}$ AS	GGQGR	GY $\underset{̲}{FG}$	1S2
	1	15S1	$\underset{̲}{C}$ ATS	VGQG	SYEQY $\underset{̲}{FG}$	2S7
	2	22S1	$\underset{̲}{C}$ ASSE	GQAGG	PQH $\underset{̲}{FG}$	1S5

No. of Clones		TCRBV		N1-D-N2	TCRBJ
Donor 1	1	1S1	$\underset{̲}{C}$ ASSV	YPRPP	QF $\underset{̲}{FG}$	2S1
	1	2S1	$\underset{̲}{C}$ SA	TKVGLGF	TDTQY $\underset{̲}{FG}$	2S3
	1	5S1	$\underset{̲}{C}$ ASSL	VEG	NTEAF $\underset{̲}{FG}$	1S1
	1	5S1	$\underset{̲}{C}$ ASSL	RAFFVD	EQF $\underset{̲}{FG}$	2S1
	4	5S2	$\underset{̲}{C}$ ASS	YGV	SGANVLT $\underset{̲}{FG}$	2S6
	1	5S6	$\underset{̲}{C}$ ASS	QGLAGV	TDTQY $\underset{̲}{FG}$	2S3
	1	5S6	$\underset{̲}{C}$ ASS	QGLAGI	TDTQY $\underset{̲}{FG}$	2S3
	1	5S6	$\underset{̲}{C}$ ASS	RDSK	NTGELF $\underset{̲}{FG}$	2S2
	1	5S6	$\underset{̲}{C}$ ASS	SGLAVSG	DTQY $\underset{̲}{FG}$	2S3
	1	13S3	$\underset{̲}{C}$ AS	TPDSFMA	T $\underset{̲}{FG}$	1S2
Patient 1	1	2S1	$\underset{̲}{C}$ SAR	GAGART	PLH $\underset{̲}{FG}$	1S6
	1	4S1	$\underset{̲}{C}$ SVE	ERRRR	ETQY $\underset{̲}{FG}$	2S5
	2	4S1	$\underset{̲}{C}$ SV	VSTGAGTAA	EQY $\underset{̲}{FG}$	2S7
	3	5S6	$\underset{̲}{C}$ ASS	QGLAGV	TDTQY $\underset{̲}{FG}$	2S3
	1	10S1	$\underset{̲}{C}$ ASS	PRGGSSLG	TDTQY $\underset{̲}{FG}$	2S3
	1	11S1	$\underset{̲}{C}$ ASS	PMGQIP	EQY $\underset{̲}{FG}$	2S7
	1	13S3	$\underset{̲}{C}$ ASSE	IFVKGADP	TDTQY $\underset{̲}{FG}$	2S3
Donor 2	1	1S1	$\underset{̲}{C}$ ASSV	ATGQ	QETQY $\underset{̲}{FG}$	2S5
	1	2S1	$\underset{̲}{C}$ SA	TGTGKS	EQY $\underset{̲}{FG}$	2S7
	1	5S1	$\underset{̲}{C}$ ASSL	EVH	NYGYT $\underset{̲}{FG}$	1S2
	2	5S1	$\underset{̲}{C}$ ASSL	AGS	SYNEQF $\underset{̲}{FG}$	2S1
	2	5S1	$\underset{̲}{C}$ ASS	SDGQGR	PQH $\underset{̲}{FG}$	2S5
	2	5S1	$\underset{̲}{C}$ AS	GFTAGVS	ETQY $\underset{̲}{FG}$	2S5
	1	5S6	$\underset{̲}{C}$ ASS	SGLAGVS	DTQY $\underset{̲}{FG}$	2S3
	1	6S5	$\underset{̲}{C}$ ASS	FRREP	SYNEQ $\underset{̲}{FG}$	2S1
	1	8S1/2	$\underset{̲}{C}$ ASSL	GRGG	TDTQY $\underset{̲}{FG}$	2S3
	1	8S1/2	$\underset{̲}{C}$ ASSL	GQAI	GYT $\underset{̲}{FG}$	1S2
	3	8S1/2	$\underset{̲}{C}$ ASS	SSGKH	EQF $\underset{̲}{FG}$	2S1
	1	8S1/2	$\underset{̲}{C}$ AS	RWGA	EAF $\underset{̲}{FG}$	1S1
	11	21S3	$\underset{̲}{C}$ ASS	FTRK	NTEAF $\underset{̲}{FG}$	1S1
Patient 2	1	2S1	$\underset{̲}{C}$ SAR	DKNAA	QF $\underset{̲}{FG}$	2S1
	2	4S1	$\underset{̲}{C}$ SV	QRLD	TIY $\underset{̲}{FG}$	1S3
	11	6S1	$\underset{̲}{C}$ ASS	QQLGGA	DTQY $\underset{̲}{FG}$	2S3
	3	6S7a	$\underset{̲}{C}$ ASS	PALAGGP	YEQY $\underset{̲}{FG}$	2S7
	2	13S3	$\underset{̲}{C}$ AS	GGQGR	GY $\underset{̲}{FG}$	1S2
	1	15S1	$\underset{̲}{C}$ ATS	VGQG	SYEQY $\underset{̲}{FG}$	2S7
	2	22S1	$\underset{̲}{C}$ ASSE	GQAGG	PQH $\underset{̲}{FG}$	1S5

Summary of the complete results of the DNA sequencing. Listed are the TCRBV/BD/BJ usage of the TT-specific T-cell clones, as well as the CDR3 region of the β chain. Nomenclature according to Arden et al51 (TCRBV) and Toyonaga et al52 (TCRBJ). When TCRBD was determined, the amino acids encoded by the D gene segment are given in bold type. The underlined CDR3 amino acids denote the boundaries of the CDR3 region on the TCRBV and TCRBJ side, respectively.

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