Phenotype of Plasma Cells From 10 RPs
| . | RP1 . | RP2* . | RP3 . | RP4 . | RP5 . | RP6 . | RP7 . | RP8 . | RP9 . | RP10 . | Total (n = 10) . |
|---|---|---|---|---|---|---|---|---|---|---|---|
| % of cells expressing | |||||||||||
| CD38+++ | 30% | 15% | 11% | 20% | 89% | 37% | 87% | 27% | 13% | 8% | +10/10 |
| κ | ++ (16%) | ++ (6%) | ND | ++ (16%) | ++ (63%) | ++ (20%) | ++ (49%) | ++ (14%) | ++ (8%) | +++ (7%) | +9/9 |
| λ | +++ (15%) | +++ (8%) | ND | +++ (4%) | +++ (28%) | +++ (17%) | +++ (40%) | +++ (13%) | +++ (4%) | +++ (3%) | +9/9 |
| CD138 | ++ (24%) | +++ (17%) | +++ (6%) | +++ (14%) | +++ (50%) | +++ (23%) | +++ (20%) | ++ (9%) | +++ (7%) | +++ (6%) | +10/10 |
| Phenotype of CD138+cells | |||||||||||
| CD11a | + (90%) | + | ND | ND | + | ND | ND | ND | ND | ND | +3/3 |
| CD19 | + | + | ND | + | ++ | + | + | + | + | + | +9/9 |
| CD28 | + (7%) | − | + (7%) | ++ (12%) | + (3%) | − | + (1%) | − | + (2%) | ND | +6/9† |
| CD40 | + | + | ND | ± | + | + | + | ND | + | ND | +7/7 |
| CD45 | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +10/10 |
| CD49e | ND | ND | ND | − | − | − | − | ND | − | ND | −5/5 |
| CD56 | − | − | − | − | − | − | − | − | − | ND | −9/9 |
| CD80 | ND | ND | ND | ND | ND | ± | ± | ± | ± | ND | ±4/4 |
| CD86 | ND | ND | ND | ND | ND | + | + | ++ | + | ND | +4/4 |
| HLA-DR | ND | ND | ND | ND | + | ND | ND | + | + | ND | +3/3 |
| Apo2.7 | ND | ND | ND | ND | ND | − | − | − | − | ND | −4/4 |
| . | RP1 . | RP2* . | RP3 . | RP4 . | RP5 . | RP6 . | RP7 . | RP8 . | RP9 . | RP10 . | Total (n = 10) . |
|---|---|---|---|---|---|---|---|---|---|---|---|
| % of cells expressing | |||||||||||
| CD38+++ | 30% | 15% | 11% | 20% | 89% | 37% | 87% | 27% | 13% | 8% | +10/10 |
| κ | ++ (16%) | ++ (6%) | ND | ++ (16%) | ++ (63%) | ++ (20%) | ++ (49%) | ++ (14%) | ++ (8%) | +++ (7%) | +9/9 |
| λ | +++ (15%) | +++ (8%) | ND | +++ (4%) | +++ (28%) | +++ (17%) | +++ (40%) | +++ (13%) | +++ (4%) | +++ (3%) | +9/9 |
| CD138 | ++ (24%) | +++ (17%) | +++ (6%) | +++ (14%) | +++ (50%) | +++ (23%) | +++ (20%) | ++ (9%) | +++ (7%) | +++ (6%) | +10/10 |
| Phenotype of CD138+cells | |||||||||||
| CD11a | + (90%) | + | ND | ND | + | ND | ND | ND | ND | ND | +3/3 |
| CD19 | + | + | ND | + | ++ | + | + | + | + | + | +9/9 |
| CD28 | + (7%) | − | + (7%) | ++ (12%) | + (3%) | − | + (1%) | − | + (2%) | ND | +6/9† |
| CD40 | + | + | ND | ± | + | + | + | ND | + | ND | +7/7 |
| CD45 | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +10/10 |
| CD49e | ND | ND | ND | − | − | − | − | ND | − | ND | −5/5 |
| CD56 | − | − | − | − | − | − | − | − | − | ND | −9/9 |
| CD80 | ND | ND | ND | ND | ND | ± | ± | ± | ± | ND | ±4/4 |
| CD86 | ND | ND | ND | ND | ND | + | + | ++ | + | ND | +4/4 |
| HLA-DR | ND | ND | ND | ND | + | ND | ND | + | + | ND | +3/3 |
| Apo2.7 | ND | ND | ND | ND | ND | − | − | − | − | ND | −4/4 |
The phenotype of plasma cells was defined in double staining with CD138. The intensity level was defined as indicated in Materials and Methods. CD38, intracytoplasmic κ and λ, and CD138+cells were determined in the total population. The phenotype of plasma cells was defined in the CD138+ cell population.
Immunological staining was performed after cells were cultured for 72 hours at 37°C in RPMI plus 10% FCS.
CD28 was either not expressed or in a minor subpopulation of plasma cells.