Anti-Mouse Monoclonal Antibodies Used in This Work
| Antibody Clone . | Specificity . | Isotype . | Affinity (Ka; M−1)* . | Saturation Level† . | Source (ref ) . |
|---|---|---|---|---|---|
| Mc10-6A5 | BCL1 Id | Ratγ2a | 1 × 109 | 0.9 × 105 | Tenovus (36) |
| Mc39-16 | Control‡ | Ratγ2a | — | — | Tenovus (35) |
| 1D3 | CD19 | Ratγ2a | 3 × 108 | 0.4 × 105 | (37) |
| NIMR6 | CD22 | Ratγ1 | 1.2 × 108 | 0.6 × 105 | (38) |
| TI2-3 | MHCII | Ratγ1 | 5.3 × 108 | 1.8 × 105 | Tenovus (34) |
| N22 | MHCII | Hamster IgG | ND | ND | ATCC |
| Antibody Clone . | Specificity . | Isotype . | Affinity (Ka; M−1)* . | Saturation Level† . | Source (ref ) . |
|---|---|---|---|---|---|
| Mc10-6A5 | BCL1 Id | Ratγ2a | 1 × 109 | 0.9 × 105 | Tenovus (36) |
| Mc39-16 | Control‡ | Ratγ2a | — | — | Tenovus (35) |
| 1D3 | CD19 | Ratγ2a | 3 × 108 | 0.4 × 105 | (37) |
| NIMR6 | CD22 | Ratγ1 | 1.2 × 108 | 0.6 × 105 | (38) |
| TI2-3 | MHCII | Ratγ1 | 5.3 × 108 | 1.8 × 105 | Tenovus (34) |
| N22 | MHCII | Hamster IgG | ND | ND | ATCC |
Abbreviations: ND, not determined; ATCC, American Tissue Culture Collection, Rockville, MD.
Estimates of the Ka values were calculated from binding curves40 by determining the concentration of antibody required to achieve half-maximum binding capacity.
Estimates of the average number antibody molecules bound to each BCL1 cell when the 125I-MoAb-binding curve had reach saturation.40
Control MoAb was directed against the Id of the A31 tumor and did not react with BCL1.