TPO mRNA Expression in Right and Left Liver Lobe, Kidney, Spleen, Lung, and Bone Marrow of Human Fetuses and Preterm Neonates
| . | Pos. (%) . | Concentration of TPO mRNA (amol/μg RNA) . | Tissue-Specific TPO mRNA (amol/g tissue) . | ||
|---|---|---|---|---|---|
| Range . | Median . | Range . | Median . | ||
| Liver, right lobe | 100 | 0.04-8.25 | 0.210 | 50-17,799 | 446 |
| n = 26 | |||||
| Liver, left lobe | 100 | 0.02-2.94 | 0.227 | 33-5,337 | 505 |
| n = 25 | |||||
| Kidney | 100 | 0.01-1.04 | 0.124 | 5-1,817 | 134 |
| n = 21 | |||||
| Spleen | 88 | 0.04-1.04 | 0.217 | 21-1,454 | 252 |
| n = 25 | |||||
| Lung | 76 | 0.03-0.31 | 0.143 | 13-214 | 55 |
| n = 17 | |||||
| Bone marrow | 84 | 0.04-0.65 | 0.214 | ND | ND |
| n = 19 | |||||
| . | Pos. (%) . | Concentration of TPO mRNA (amol/μg RNA) . | Tissue-Specific TPO mRNA (amol/g tissue) . | ||
|---|---|---|---|---|---|
| Range . | Median . | Range . | Median . | ||
| Liver, right lobe | 100 | 0.04-8.25 | 0.210 | 50-17,799 | 446 |
| n = 26 | |||||
| Liver, left lobe | 100 | 0.02-2.94 | 0.227 | 33-5,337 | 505 |
| n = 25 | |||||
| Kidney | 100 | 0.01-1.04 | 0.124 | 5-1,817 | 134 |
| n = 21 | |||||
| Spleen | 88 | 0.04-1.04 | 0.217 | 21-1,454 | 252 |
| n = 25 | |||||
| Lung | 76 | 0.03-0.31 | 0.143 | 13-214 | 55 |
| n = 17 | |||||
| Bone marrow | 84 | 0.04-0.65 | 0.214 | ND | ND |
| n = 19 | |||||
TPO cDNA was quantified by competitive PCR, and mRNA concentrations were calculated considering the RT efficiency, the amount of reverse transcribed RNA, and the concentration of total RNA per gram tissue. Median and range include RT-PCR positive samples only.
Abbreviations: n, total number of tissue samples; Pos., fraction of positive samples in TPO RT-PCR; ND, not determined.