PCR Primers Specific for GAPDH and TPO cDNA, to Generate the T7 RNA Polymerase Template (5′ T7-TPO and 3′ dT-TPO primers) and the TPO Competitor DNA (5′ TPO composite and 3′ TPO primers)
| . | Nucleotide Sequence . |
|---|---|
| 5′ GAPDH primer | 5′-ATC ATC CCT GCC TCT ACT GG-3′ |
| 3′ GAPDH primer | 5′-TGG GTG TCG CTG TTG AAG TC-3′ |
| 5′ TPO primer | 5′-CGT TTC CTG ATG CTT GTA GG-3′ |
| 3′ TPO primer | 5′-GAA GGA GAA TAT CCA GGC TG-3′ |
| 5′ T7-TPO primer | 5′-GGA TCC TAA TAC GAC TCA CTA TAG GGA GGC GTT TCC TGA TGC TTG TAG G-3′ |
| 3′ TPO-dT primer | 5′-TTT TTT TTT TTT TTT TTT TTG AAG GAG AAT ATC CAG GCT G -3′ |
| 5′ TPO composite primer | 5′-CGT TTC CTG ATG CTT GTA GG = C AAA CAG GAC TTC TGG ATT G-3′ |
| . | Nucleotide Sequence . |
|---|---|
| 5′ GAPDH primer | 5′-ATC ATC CCT GCC TCT ACT GG-3′ |
| 3′ GAPDH primer | 5′-TGG GTG TCG CTG TTG AAG TC-3′ |
| 5′ TPO primer | 5′-CGT TTC CTG ATG CTT GTA GG-3′ |
| 3′ TPO primer | 5′-GAA GGA GAA TAT CCA GGC TG-3′ |
| 5′ T7-TPO primer | 5′-GGA TCC TAA TAC GAC TCA CTA TAG GGA GGC GTT TCC TGA TGC TTG TAG G-3′ |
| 3′ TPO-dT primer | 5′-TTT TTT TTT TTT TTT TTT TTG AAG GAG AAT ATC CAG GCT G -3′ |
| 5′ TPO composite primer | 5′-CGT TTC CTG ATG CTT GTA GG = C AAA CAG GAC TTC TGG ATT G-3′ |
In the sequence of the 5′ TPO composite primer, the position of the 86-bp deletion with respect to the TPO cDNA sequence8is indicated (=).