The Ability of FDC to Inhibit T-Cell Proliferation in the Absence of Cellular Contact
| Cells Present in* . | % Proliferative Response (±SEM)† . | |
|---|---|---|
| Insert . | Well . | |
| T cells + FDC | — | 37 ± 3.7 |
| T cells | FDC | 56 ± 5.1‡ |
| T cells + B cells | — | 123 ± 22.1 |
| T cells | B cells | 103 ± 4.0 |
| Cells Present in* . | % Proliferative Response (±SEM)† . | |
|---|---|---|
| Insert . | Well . | |
| T cells + FDC | — | 37 ± 3.7 |
| T cells | FDC | 56 ± 5.1‡ |
| T cells + B cells | — | 123 ± 22.1 |
| T cells | B cells | 103 ± 4.0 |
Assays were performed in 24-well plates fitted with cell culture inserts containing a 3-μm pore size membrane.
T cells (4 × 105) and SEB at 100 ng/mL were placed in the insert. FDCs and B cells (8 × 104) were placed either in the insert or well, as indicated. The mean proliferative responses from three independent experiments are combined and expressed as a percentage (±SEM) of the response obtained when T cells were cultured with only SEB.
Tritiated thymidine was measured during the last 24 hours of a 3-day culture. Cpm for cultures containing only T cells ranged from 1,498 to 1,658.
P < .05 using a paired t-test when compared with cultures containing T cells and FDCs in the same chamber.