Summary of the In Vivo Activity of Different Flk-1 Constructs
| Construct . | TG . | ES . | ET . | NO . |
|---|---|---|---|---|
| −4100/+299 | 11 | 0 | 3 | 8 |
| −1900/+299 | 31 | 1 | 10 | 20 |
| −640/+299 | 3 | 0 | 1 | 2 |
| −640/+299 // 3′Intron +1677/+3947 | 7 | 6 | 0 | 1 |
| −640/+299 // 3′Intron +3947/+1677 | 4 | 3 | 1 | 0 |
| −640/+299 // 3′Intron +3437/+3947 | 7 | 5 | 0 | 2 |
| −640/+254 // 3′Intron +1677/+3947 | 12 | 8 | 1 | 3 |
| −640/+137 // 3′Intron +1677/+3947 | 13 | 5 | 1 | 7 |
| tk // 3′Intron +1677/+3947 | 15 | 3 | 0 | 12 |
| −5500/+299 // Intron I+II | 3 | 2 | 0 | 1 |
| Construct . | TG . | ES . | ET . | NO . |
|---|---|---|---|---|
| −4100/+299 | 11 | 0 | 3 | 8 |
| −1900/+299 | 31 | 1 | 10 | 20 |
| −640/+299 | 3 | 0 | 1 | 2 |
| −640/+299 // 3′Intron +1677/+3947 | 7 | 6 | 0 | 1 |
| −640/+299 // 3′Intron +3947/+1677 | 4 | 3 | 1 | 0 |
| −640/+299 // 3′Intron +3437/+3947 | 7 | 5 | 0 | 2 |
| −640/+254 // 3′Intron +1677/+3947 | 12 | 8 | 1 | 3 |
| −640/+137 // 3′Intron +1677/+3947 | 13 | 5 | 1 | 7 |
| tk // 3′Intron +1677/+3947 | 15 | 3 | 0 | 12 |
| −5500/+299 // Intron I+II | 3 | 2 | 0 | 1 |
Embryos transgenic for the constructs given above were generated, and LacZ staining and genotyping were performed at E10.5 or E11.5 as described in the Materials and Methods section. Constructs are defined by the position of the promoter or intron fragments in bp relative to the transcription initiation site of the endogenous Flk-1 gene.
Abbreviations: TG, number of transgenic embryos; ES, number of embryos showing endothelial-specific staining; ET, number of embryos showing ectopic staining; NO, number of embryos showing no staining at all.