Primers Used for PCR Amplification
| Name . | Sequence . | Primer Length (bp) . | GC (%) . | Tm (°C) (0.05 mol/L Na)* . |
|---|---|---|---|---|
| p27S | 5′-CCTCTTCGGCCCGGTGGAC-3′ | 19 | 73.7 | 54.6 |
| p21AS | 5′-CCGTTTTCGACCCTGAGAG-3′ | 19 | 57.9 | 48.1 |
| p27AS | 5′-TCTGCTCCACAGAACCGGC-3′ | 19 | 63.2 | 50.3 |
| βS | 5′-AAGAGAGGCATCCTCACCCT-3′ | 20 | 55.0 | 48.7 |
| βASp27S | 5′-CCTCTTCGGCCCGGTGGACGGAAGGAAGGCTGGAAG-3′ | 36 | 66.7 | 68.5 |
| Name . | Sequence . | Primer Length (bp) . | GC (%) . | Tm (°C) (0.05 mol/L Na)* . |
|---|---|---|---|---|
| p27S | 5′-CCTCTTCGGCCCGGTGGAC-3′ | 19 | 73.7 | 54.6 |
| p21AS | 5′-CCGTTTTCGACCCTGAGAG-3′ | 19 | 57.9 | 48.1 |
| p27AS | 5′-TCTGCTCCACAGAACCGGC-3′ | 19 | 63.2 | 50.3 |
| βS | 5′-AAGAGAGGCATCCTCACCCT-3′ | 20 | 55.0 | 48.7 |
| βASp27S | 5′-CCTCTTCGGCCCGGTGGACGGAAGGAAGGCTGGAAG-3′ | 36 | 66.7 | 68.5 |
Tm was calculated using the formula: Tm (°C) = 81.5 + 16.6 (Log [molar Na+]) + 41 (%GC) − 675/primer length.