Activation of Platelet Procaspase-3 by Physiological Agonists
| Agonist . | Procaspase-3 Cleaved (%) . |
|---|---|
| No additive | 0.3 ± 1.8 |
| Thrombin (1 U/mL) | 14.8 ± 2.8 |
| Collagen (10 μg/mL) | 14.2 ± 3.9 |
| Thrombin (1 U/mL) + collagen (10 μg/mL) | 21.0 ± 7.2 |
| A23187 (3 μmol/L) | 30.2 ± 6.3 |
| Agonist . | Procaspase-3 Cleaved (%) . |
|---|---|
| No additive | 0.3 ± 1.8 |
| Thrombin (1 U/mL) | 14.8 ± 2.8 |
| Collagen (10 μg/mL) | 14.2 ± 3.9 |
| Thrombin (1 U/mL) + collagen (10 μg/mL) | 21.0 ± 7.2 |
| A23187 (3 μmol/L) | 30.2 ± 6.3 |
Platelets were incubated with agonist for 20 minutes. Procaspase-3 content was determined by immunoblotting with C31720 MoAb and quantitation of the 32-kD band. Results are expressed as the percentage of decrease (mean ± SEM of 3 experiments) relative to nonactivated starting platelets.