Table 1. Expression of HM1.24 and the... | American Society of Hematology

Table 1.

Expression of HM1.24 and the Sensitivity to Humanized Anti-HM1.24 MoAb of Neoplastic Myeloma Cells

Target Cells	HM1.24 Expression (MSFI)	Cytotoxicity (%)
Target Cells	HM1.24 Expression (MSFI)	Control Human IgG	Humanized Anti-HM1.24
ARH-77	43	2	44
IM-9	38	6	61
RPMI 8226	36	0	37
Ramos	33	0	33
U 266	30	38^*	59
HS-Sultan	21	0	31
Daudi	12	0	11
Patient no. 1	34	4	29
		1^†	25^†
Patient no. 2	33	0	21
		0^†	27^†
Patient no. 3	30	0	16
Patient no. 4	30	7	32
CHO-1	50^‡	5	38
CHO-2	20^‡	11	28
CHO-3	5^‡	7	8
CHO-4	1^‡	0	1
HEL	1^1-153	6	6

Target Cells	HM1.24 Expression (MSFI)	Cytotoxicity (%)
Target Cells	HM1.24 Expression (MSFI)	Control Human IgG	Humanized Anti-HM1.24
ARH-77	43	2	44
IM-9	38	6	61
RPMI 8226	36	0	37
Ramos	33	0	33
U 266	30	38^*	59
HS-Sultan	21	0	31
Daudi	12	0	11
Patient no. 1	34	4	29
		1^†	25^†
Patient no. 2	33	0	21
		0^†	27^†
Patient no. 3	30	0	16
Patient no. 4	30	7	32
CHO-1	50^‡	5	38
CHO-2	20^‡	11	28
CHO-3	5^‡	7	8
CHO-4	1^‡	0	1
HEL	1^1-153	6	6

HM1.24 expression was examined by flow cytometry. Cytotoxicity against tumor cells was determined by a 4-hour ⁵¹Cr-release assay using normal human PBMCs as effector cells at an E/T ratio of 50 in the presence of 1 μg/mL control human IgG or humanized anti-HM1.24 MoAb. Data represent the mean value of three independent experiments. The standard error of cytotoxicity was <10% in all target cells.

U266 is NK-sensitive.

†

Autologous PBMCs were used as effector cells.

‡

CHO transformants expressing different levels of HM1.24 antigen were used.

F1-153

HEL does not express HM1.24 antigen.

View Large

This Feature Is Available To Subscribers Only