Production of Cytokines During Induction of MUC1-Specific CTL Using Peptide-Pulsed DC
| . | No Peptide . | M1.1 Peptide . | +PADRE* . |
|---|---|---|---|
| IL-12 (pg/mL) | 43.3 ± 17 | 349 ± 140 | 786 ± 62 |
| IFN-γ (pg/mL) | 0.8 ± 1.2 | 16.8 ± 1.6 | 33 ± 9.5 |
| TNF-α (pg/mL) | 36.8 ± 5.5 | 205 ± 17 | 306 ± 21 |
| IL-10 (pg/mL) | 17.2 ± 2.2 | 24 ± 4.2 | 32 ± 1.9 |
| IL-6 (pg/mL) | 2.4 ± 1.7 | 6.5 ± 3.5 | 16 ± 4.6 |
| sIL-2R (U/mL) | 56.8 ± 8.1 | 201 ± 26 | 304 ± 39 |
| . | No Peptide . | M1.1 Peptide . | +PADRE* . |
|---|---|---|---|
| IL-12 (pg/mL) | 43.3 ± 17 | 349 ± 140 | 786 ± 62 |
| IFN-γ (pg/mL) | 0.8 ± 1.2 | 16.8 ± 1.6 | 33 ± 9.5 |
| TNF-α (pg/mL) | 36.8 ± 5.5 | 205 ± 17 | 306 ± 21 |
| IL-10 (pg/mL) | 17.2 ± 2.2 | 24 ± 4.2 | 32 ± 1.9 |
| IL-6 (pg/mL) | 2.4 ± 1.7 | 6.5 ± 3.5 | 16 ± 4.6 |
| sIL-2R (U/mL) | 56.8 ± 8.1 | 201 ± 26 | 304 ± 39 |
For CTL induction, DC were pulsed with the synthetic MUC1 peptide M1.1 and incubated with autologous PBMNC in RP10 medium. To analyze the effect of a helper epitope on antigen-specific CTL induction, DC were further incubated with a Pan-HLA-DR binding peptide PADRE in addition to the HLA-A2–binding antigenic peptides. After 5 days of culture, cytokine concentrations in the supernatants were determined using commercially available ELISAs. Experiments were performed in triplicates and the results are expressed as the mean ± SD.