Collagen-Mediated Changes in [Ca2+]i in Mouse Megakaryocytes in the Presence of Tyrosine Kinase Inhibitors and in Kinase-Deficient Megakaryocytes
| . | Peak [Ca2+]i Value (nmol/L; mean ± SEM) . | Increase in [Ca2+]i(nmol/L; mean ± SEM) . | n . | |
|---|---|---|---|---|
| Resting . | Collagen (100 μg/mL) . | |||
| Control | 66 ± 7 | 217 ± 19 | 161 ± 17 | 10 |
| Syk SH2 domains | 56 ± 7 | 188 ± 11 | 133 ± 11* | 10 |
| Syk-deficient | 73 ± 6 | 179 ± 10 | 114 ± 12* | 6 |
| 10 μmol/L PP1 | 67 ± 13 | 201 ± 18 | 136 ± 8* | 9 |
| Fyn-deficient | 77 ± 17 | 183 ± 26 | 106 ± 27 | 4 |
| 3 μmol/L Staurosporine | 74 ± 19 | 224 ± 33 | 155 ± 37 | 8 |
| . | Peak [Ca2+]i Value (nmol/L; mean ± SEM) . | Increase in [Ca2+]i(nmol/L; mean ± SEM) . | n . | |
|---|---|---|---|---|
| Resting . | Collagen (100 μg/mL) . | |||
| Control | 66 ± 7 | 217 ± 19 | 161 ± 17 | 10 |
| Syk SH2 domains | 56 ± 7 | 188 ± 11 | 133 ± 11* | 10 |
| Syk-deficient | 73 ± 6 | 179 ± 10 | 114 ± 12* | 6 |
| 10 μmol/L PP1 | 67 ± 13 | 201 ± 18 | 136 ± 8* | 9 |
| Fyn-deficient | 77 ± 17 | 183 ± 26 | 106 ± 27 | 4 |
| 3 μmol/L Staurosporine | 74 ± 19 | 224 ± 33 | 155 ± 37 | 8 |
The table indicates the resting and peak [Ca2+]i (in nanomoles per liter) in a series of FURA-2–injected mouse megakaryocytes after treatment with collagen (100 μg/mL) and the effect of injection of p72syk SH2 domains, pretreatment with PP1 or staurosporine, or in the absence of the p72syk or p59fyn in megakaryocytes from protein-deficient mice. Results are indicated as the mean ± SE mean of the number of cells indicated in the right-hand column. Resting [Ca2+]i values were taken immediately before the addition of agonist, and the increase in [Ca2+]i was determined as the difference between this value and the peak [Ca2+]imeasured after the addition of agonist.
P < .05 v control using the one-tailed independent Student’s t-test.