VEGF-A Concentrations in Supernatants of Primary T Cells Under Different Conditions of Stimulation
| Stimuli . | VEGF-A (pg/mL) . | ||
|---|---|---|---|
| Person 1 . | Person 2 . | Person 3 . | |
| Buffer | 11.4 ± 2.9 | 15.3 ± 0.8 | 21.9 ± 1.6 |
| TCM (25%)* | 15.6 ± 3.8 | 32.3 ± 3.8 | 30.5 ± 2.1 |
| HIV-TCM (25%)* | 34.6 ± 8.3 | 45.7 ± 8.3 | 48.6 ± 8.4 |
| TNFα (250 pg/mL) | 14.4 ± 1.3 | 20.1 ± 0.8 | 24.9 ± 5.8 |
| IFNγ (1 U/mL) | 13.5 ± 2.5 | 21.9 ± 0.8 | 24.0 ± 1.3 |
| IL-1β (875 pg/mL) | 18.3 ± 1.6 | 27.2 ± 1.7 | 27.9 ± 0.8 |
| IL-6 (200 U/mL) | 14.7 ± 1.7 | 22.2 ± 5.0 | 24.0 ± 0.4 |
| SH-TCM | 70.1 ± 12.9 | 51.8 ± 12.9 | 56.7 ± 1.7 |
| Stimuli . | VEGF-A (pg/mL) . | ||
|---|---|---|---|
| Person 1 . | Person 2 . | Person 3 . | |
| Buffer | 11.4 ± 2.9 | 15.3 ± 0.8 | 21.9 ± 1.6 |
| TCM (25%)* | 15.6 ± 3.8 | 32.3 ± 3.8 | 30.5 ± 2.1 |
| HIV-TCM (25%)* | 34.6 ± 8.3 | 45.7 ± 8.3 | 48.6 ± 8.4 |
| TNFα (250 pg/mL) | 14.4 ± 1.3 | 20.1 ± 0.8 | 24.9 ± 5.8 |
| IFNγ (1 U/mL) | 13.5 ± 2.5 | 21.9 ± 0.8 | 24.0 ± 1.3 |
| IL-1β (875 pg/mL) | 18.3 ± 1.6 | 27.2 ± 1.7 | 27.9 ± 0.8 |
| IL-6 (200 U/mL) | 14.7 ± 1.7 | 22.2 ± 5.0 | 24.0 ± 0.4 |
| SH-TCM | 70.1 ± 12.9 | 51.8 ± 12.9 | 56.7 ± 1.7 |
VEGF-A concentrations in supernatants produced from 5 × 105 primary T cells after 5 days of stimulation were determined in duplicate by ELISA.
Corrected VEGF-A concentrations are presented. The concentration of VEGF-A present in TCM and HIV-TCM used for induction in the respective experiments has been subtracted.