Involvement of iNOS in the Cytotoxic Activity of IL-2–Treated-PB-NK Cells
| E/T Ratios . | % Specific Lysis . | |||
|---|---|---|---|---|
| 100:1 . | 50:1 . | 25:1 . | 12.5:1 . | |
| Control | 68 | 50 | 35 | 28 |
| +L-NMMA | 31.2 | 22.6 | 17.5 | 12.1 |
| +AG | 30.3 | 26.1 | 17 | 10.4 |
| +AS | 31 | 23.3 | 18.1 | 9.1 |
| +Scr | 63 | 51 | 32.7 | 26.2 |
| % DNA Fragmentation | ||||
| Control | 40.3 | 31 | 20.2 | 16.1 |
| +L-NMMA | 31.1 | 24.3 | 15.3 | 10 |
| +AG | 30 | 22.7 | 12.7 | 11.1 |
| +AS | 29.1 | 23 | 13.1 | 11.4 |
| +Scr | 41.2 | 30.7 | 22.1 | 17.4 |
| E/T Ratios . | % Specific Lysis . | |||
|---|---|---|---|---|
| 100:1 . | 50:1 . | 25:1 . | 12.5:1 . | |
| Control | 68 | 50 | 35 | 28 |
| +L-NMMA | 31.2 | 22.6 | 17.5 | 12.1 |
| +AG | 30.3 | 26.1 | 17 | 10.4 |
| +AS | 31 | 23.3 | 18.1 | 9.1 |
| +Scr | 63 | 51 | 32.7 | 26.2 |
| % DNA Fragmentation | ||||
| Control | 40.3 | 31 | 20.2 | 16.1 |
| +L-NMMA | 31.1 | 24.3 | 15.3 | 10 |
| +AG | 30 | 22.7 | 12.7 | 11.1 |
| +AS | 29.1 | 23 | 13.1 | 11.4 |
| +Scr | 41.2 | 30.7 | 22.1 | 17.4 |
Cytotoxic assay was performed as described in Materials and Methods at the indicated E/T ratios. YAC-1 cells were used as targets. Where indicated, the cytotoxicity assay was performed with NK cells pretreated with AS or Scr or in the presence of NOS inhibitors, L-NMMA (500 μmol/L) and AG (50 μmol/L). Each value represents the mean of triplicate determinations from one representative experiment. SD was less than 5%. Similar results were obtained from two other experiments.