iNOS Activity in IL-2–Treated PB-NK Cells and S-NK
| Treatment . | pmol Citrulline/min/mg Protein . | |||
|---|---|---|---|---|
| PB-NK . | S-NK . | |||
| +Ca/CAM . | −Ca/CAM + W13 . | +Ca/CAM . | −Ca/CAM + W13 . | |
| Control | 2.5 ± 0.9 | 3.0 ± 0.7 | 4.3 ± 0.3 | 5.6 ± 1.1 |
| IL-2 | 55.2 ± 3.6 | 57.1 ± 4.9 | 97.1 ± 7.7 | 95.3 ± 4.6 |
| L-NMMA | 2.7 ± 0.4 | 2.8 ± 0.2 | 3.3 ± 0.8 | 4.8 ± 0.6 |
| AG | 2.9 ± 0.8 | 2.7 ± 0.4 | 3.0 ± 0.9 | 5.7 ± 0.4 |
| +L-NMMA | 3.0 ± 0.8 | 2.7 ± 0.2 | 5.3 ± 0.9 | 4.1 ± 0.6 |
| +AG | 2.9 ± 0.1 | 3.1 ± 0.4 | 3.3 ± 0.1 | 3.5 ± 0.6 |
| Treatment . | pmol Citrulline/min/mg Protein . | |||
|---|---|---|---|---|
| PB-NK . | S-NK . | |||
| +Ca/CAM . | −Ca/CAM + W13 . | +Ca/CAM . | −Ca/CAM + W13 . | |
| Control | 2.5 ± 0.9 | 3.0 ± 0.7 | 4.3 ± 0.3 | 5.6 ± 1.1 |
| IL-2 | 55.2 ± 3.6 | 57.1 ± 4.9 | 97.1 ± 7.7 | 95.3 ± 4.6 |
| L-NMMA | 2.7 ± 0.4 | 2.8 ± 0.2 | 3.3 ± 0.8 | 4.8 ± 0.6 |
| AG | 2.9 ± 0.8 | 2.7 ± 0.4 | 3.0 ± 0.9 | 5.7 ± 0.4 |
| +L-NMMA | 3.0 ± 0.8 | 2.7 ± 0.2 | 5.3 ± 0.9 | 4.1 ± 0.6 |
| +AG | 2.9 ± 0.1 | 3.1 ± 0.4 | 3.3 ± 0.1 | 3.5 ± 0.6 |
Isolated PB-NK or S-NK cells (106/mL) were incubated in control medium or in medium containing IL-2 (100 U/mL) for 18 hours at 37°C in 5% CO2 in air in the presence or absence of L-NMMA (500 μmol/L) or AG (50 μmol/L). Cell extracts were assayed for the enzymatic conversion of [3H]-L–arginine to [3H]-L–citrulline as described in Materials and Methods. The assay was performed in the presence or absence of CaCl2(0.6 mmol/L) and CAM (0.1 μmol/L). Data are means ± SD from one representative experiment in triplicate. Similar results were obtained in two other experiments.