Immunophenotyping Panels for ALL
| Panel . | Purpose . | Label-CD Cluster . |
|---|---|---|
| 1 | Defines leukocyte gate | FL-CD45 |
| PE-CD14 | ||
| TC-HLADr | ||
| 2 | Defines major T-cell subsets and their malignant counterparts | FL-CD3 PE-CD4 |
| TC-CD8 | ||
| 3 | Defines uncommitted progenitor cells (34+, 38−, DR−) from committed progenitor cells (34+, 38+, DR−) | FL-CD38 PE-CD34 TC-HLADR |
| 4 | Detects CD10 on B cells, or T cells; detects CLL (19+, 5+) | FL-CD5 PE-CD19 |
| TC-CD10 | ||
| 5 | Detects immature T- or B-cell malignancies | FL-CD5 |
| PE-CD34 | ||
| TC-CD19 | ||
| 6 | Helps distinguish B-ALL from B-lymphoproliferative disorders (11c+) | FL-CD20 PE-CD11c |
| TC-CD22 | ||
| 7 | Detects CD19+ B-myeloid cells | FL-CD33 |
| PE-CD13 | ||
| TC-CD19 | ||
| 8 | Verifies or excludes CD19 expression on T-ALL | FL-CD7 PE-CD19 TC-CD2 |
| 9 | Detects surface Ig expression on B cells | FL-IgD |
| PE-IgM | ||
| TC-CD20 | ||
| 10 | Detects light-chain clonal excess for B-cell malignancies | FL-κ PE-λ |
| TC-CD20 | ||
| 11 | Isotype control for nonspecific binding | FL-IgG1 |
| PE-IgG2a | ||
| TC-IgG2b |
| Panel . | Purpose . | Label-CD Cluster . |
|---|---|---|
| 1 | Defines leukocyte gate | FL-CD45 |
| PE-CD14 | ||
| TC-HLADr | ||
| 2 | Defines major T-cell subsets and their malignant counterparts | FL-CD3 PE-CD4 |
| TC-CD8 | ||
| 3 | Defines uncommitted progenitor cells (34+, 38−, DR−) from committed progenitor cells (34+, 38+, DR−) | FL-CD38 PE-CD34 TC-HLADR |
| 4 | Detects CD10 on B cells, or T cells; detects CLL (19+, 5+) | FL-CD5 PE-CD19 |
| TC-CD10 | ||
| 5 | Detects immature T- or B-cell malignancies | FL-CD5 |
| PE-CD34 | ||
| TC-CD19 | ||
| 6 | Helps distinguish B-ALL from B-lymphoproliferative disorders (11c+) | FL-CD20 PE-CD11c |
| TC-CD22 | ||
| 7 | Detects CD19+ B-myeloid cells | FL-CD33 |
| PE-CD13 | ||
| TC-CD19 | ||
| 8 | Verifies or excludes CD19 expression on T-ALL | FL-CD7 PE-CD19 TC-CD2 |
| 9 | Detects surface Ig expression on B cells | FL-IgD |
| PE-IgM | ||
| TC-CD20 | ||
| 10 | Detects light-chain clonal excess for B-cell malignancies | FL-κ PE-λ |
| TC-CD20 | ||
| 11 | Isotype control for nonspecific binding | FL-IgG1 |
| PE-IgG2a | ||
| TC-IgG2b |