cDNA samples from immature (−MCM) or mature (+MCM) DC were used in light-cycle PCR reactions as described in Materials and Methods. The amount of product for each PCR reaction was calculated from the incorporation of SYBR green, and standardized to actin for individual cDNA samples. Results are expressed as the fold change upon addition of MCM. These data are representative of duplicates for one cDNA sample and repeats upon three independent sets of cDNA.