Immunophenotypic, Molecular, and Cytogenetic Results for the Five B-Cell ALK-Positive ALCL Cases
| Case* . | CD20 . | CD3 . | IgH/L . | CD30 . | Molecular . | RT-PCR . | Cytogenetics . |
|---|---|---|---|---|---|---|---|
| 1 | + | − | NA | + | VJ-C | NA | NA |
| 2 | + | − | IgMλ | − | NA | NA | NA |
| 3 | + | − | − | + | NA | NA | NA |
| 4 | + | − | IgMλ | + | NA | NA | NA |
| 5 | − | − | IgAκ | − | VJ-C | − | t(2;5) |
| Case* . | CD20 . | CD3 . | IgH/L . | CD30 . | Molecular . | RT-PCR . | Cytogenetics . |
|---|---|---|---|---|---|---|---|
| 1 | + | − | NA | + | VJ-C | NA | NA |
| 2 | + | − | IgMλ | − | NA | NA | NA |
| 3 | + | − | − | + | NA | NA | NA |
| 4 | + | − | IgMλ | + | NA | NA | NA |
| 5 | − | − | IgAκ | − | VJ-C | − | t(2;5) |
Abbreviations: IgH/L, immunoglobulin heavy- (IgH) and light-chain immunophenotypic analysis using either paraffin section and/or frozen-section techniques; molecular, polymerase chain reaction for IgH and T-cell receptor gamma; VJ-C, clonal immunoglobulin variable region rearrangement; RT-PCR, reverse transcriptase PCR for NPM-ALK rearrangements; NA, not available; (−), negative.
All five cases showed ALK expression in both the nucleus and cytoplasm.