Effect of Jaspamide on the Proliferation of HL-60 Cells
| Treatment . | Incubation Time . | |||
|---|---|---|---|---|
| 24 h . | 48 h . | |||
| Cell No./Well . | cpm . | Cell No./Well . | cpm . | |
| Nil | 1.5 ± 0.1 | 5,153 ± 129 | 2.6 ± 0.2 | 7,078 ± 846 |
| DMSO 0.01% | 1.4 ± 0.01 | 4,984 ± 413 | 2.0 ± 0.1 | 6,452 ± 542 |
| Jaspamide 10−8 mol/L | 1.2 ± 0.1 | 4,729 ± 318 | 2.2 ± 0.1 | 7,078 ± 846 |
| Jaspamide 5 × 10−8 mol/L | 1.1 ± 0.06 | 3,629 ± 1,125* | 1.2 ± 0.1* | 4,386 ± 348* |
| Jaspamide 10−7 mol/L | 1.0 ± 0.14* | 2,783 ± 181* | 0.36 ± 0.06* | 1,625 ± 576* |
| Treatment . | Incubation Time . | |||
|---|---|---|---|---|
| 24 h . | 48 h . | |||
| Cell No./Well . | cpm . | Cell No./Well . | cpm . | |
| Nil | 1.5 ± 0.1 | 5,153 ± 129 | 2.6 ± 0.2 | 7,078 ± 846 |
| DMSO 0.01% | 1.4 ± 0.01 | 4,984 ± 413 | 2.0 ± 0.1 | 6,452 ± 542 |
| Jaspamide 10−8 mol/L | 1.2 ± 0.1 | 4,729 ± 318 | 2.2 ± 0.1 | 7,078 ± 846 |
| Jaspamide 5 × 10−8 mol/L | 1.1 ± 0.06 | 3,629 ± 1,125* | 1.2 ± 0.1* | 4,386 ± 348* |
| Jaspamide 10−7 mol/L | 1.0 ± 0.14* | 2,783 ± 181* | 0.36 ± 0.06* | 1,625 ± 576* |
Cells were incubated with jaspamide for 24 and 48 hours in liquid cultures at an initial concentration of 104 HL-60 cells/200 μL. The cells were counted by trypan blue exclusion.3H-thymidine incorporation studies were performed as described in Materials and Methods. Results are the mean ± SE of three experiments performed in triplicate.
Indicated P < .05.