Chemokine Induction by Eotaxin-Stimulated Thymocytes
| Stimulus . | Donor 1 . | Donor 2 . | Donor 3 . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| IL-8 . | MIP-1β (pg/mL) . | RANTES . | IL-8 . | MIP-1β (pg/mL) . | RANTES . | IL-8 . | MIP-1β (pg/mL) . | RANTES . | |
| Eotaxin | |||||||||
| Control | 100 | 111 | 16 | 48 | 82 | 32 | 198 | 131 | 55 |
| 1 nmol/L | 129 | 1,036 | 145 | 65 | 83 | 317 | 178 | 137 | ND |
| 10 nmol/L | 146 | 915 | 156 | 65 | 857 | 314 | 235 | 141 | ND |
| 100 nmol/L | 237 | 1,260 | 177 | 106 | 1,170 | 371 | 345 | 1,840 | 65 |
| IL-2 | 2,278 | 9,964 | 697 | 5,088 | 1,342 | 200 | 8,108 | 1,289 | 600 |
| IL-7 | ND | ND | ND | 163 | 102 | 29 | 459 | 107 | 29 |
| Stimulus . | Donor 1 . | Donor 2 . | Donor 3 . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| IL-8 . | MIP-1β (pg/mL) . | RANTES . | IL-8 . | MIP-1β (pg/mL) . | RANTES . | IL-8 . | MIP-1β (pg/mL) . | RANTES . | |
| Eotaxin | |||||||||
| Control | 100 | 111 | 16 | 48 | 82 | 32 | 198 | 131 | 55 |
| 1 nmol/L | 129 | 1,036 | 145 | 65 | 83 | 317 | 178 | 137 | ND |
| 10 nmol/L | 146 | 915 | 156 | 65 | 857 | 314 | 235 | 141 | ND |
| 100 nmol/L | 237 | 1,260 | 177 | 106 | 1,170 | 371 | 345 | 1,840 | 65 |
| IL-2 | 2,278 | 9,964 | 697 | 5,088 | 1,342 | 200 | 8,108 | 1,289 | 600 |
| IL-7 | ND | ND | ND | 163 | 102 | 29 | 459 | 107 | 29 |
Purified human thymocytes were cultured overnight in 6-well costar plates in the presence or absence of increasing concentrations of eotaxin or 10 ng/mL of human recombinant IL-2 or IL-7. Supernatants were collected and analyzed for various cytokines and chemokines as described in Materials and Methods, and results of chemokine analyses are shown for three separate donors.
Abbreviation: ND, not determined.