Angiogenic Response to the Plasma Cell CM Assessed by the CAM Gelatin Sponge Assay
| Sample . | No. of Samples3-150 . | Microvessel Area as mm2 × 10−2 Mean ± SD (range) . | Angiogenic Samples3-152 No. (%) . |
|---|---|---|---|
| Negative control medium3-151 | 4 | 0.53 ± 0.15 (0.25-0.75) | 0 |
| Positive control medium | 4 | 2.97 ± 1.24 (0.62-4.62) | 4 (100) |
| Plasma cell CM from active MM patients | |||
| Total | 26 | 2.48 ± 1.24 (0.5-4.07)3-153 | 20 (76)3-153 |
| Diagnosis | 10 | 2.74 ± 1.24 (0.5-4.07)3-153 | 8 (80)3-153 |
| Relapse | 10 | 2.11 ± 1.27 (0.5-3.87)3-153 | 7 (70)3-153 |
| Progression | 6 | 2.70 ± 1.08 (0.5-3.87)3-153 | 5 (83)3-153 |
| Plasma cell CM from nonactive MM patients | |||
| Total | 18 | 0.91 ± 0.61 (0.37-2.75) | 6 (33) |
| Response | 11 | 1.00 ± 0.69 (0.37-2.75) | 4 (36) |
| Plateau | 7 | 0.77 ± 0.44 (0.37-2.25) | 2 (28) |
| Plasma cell CM from MGUS patients | |||
| Total | 20 | 0.90 ± 0.81 (0.25-3.37) | 4 (20) |
| Sample . | No. of Samples3-150 . | Microvessel Area as mm2 × 10−2 Mean ± SD (range) . | Angiogenic Samples3-152 No. (%) . |
|---|---|---|---|
| Negative control medium3-151 | 4 | 0.53 ± 0.15 (0.25-0.75) | 0 |
| Positive control medium | 4 | 2.97 ± 1.24 (0.62-4.62) | 4 (100) |
| Plasma cell CM from active MM patients | |||
| Total | 26 | 2.48 ± 1.24 (0.5-4.07)3-153 | 20 (76)3-153 |
| Diagnosis | 10 | 2.74 ± 1.24 (0.5-4.07)3-153 | 8 (80)3-153 |
| Relapse | 10 | 2.11 ± 1.27 (0.5-3.87)3-153 | 7 (70)3-153 |
| Progression | 6 | 2.70 ± 1.08 (0.5-3.87)3-153 | 5 (83)3-153 |
| Plasma cell CM from nonactive MM patients | |||
| Total | 18 | 0.91 ± 0.61 (0.37-2.75) | 6 (33) |
| Response | 11 | 1.00 ± 0.69 (0.37-2.75) | 4 (36) |
| Plateau | 7 | 0.77 ± 0.44 (0.37-2.25) | 2 (28) |
| Plasma cell CM from MGUS patients | |||
| Total | 20 | 0.90 ± 0.81 (0.25-3.37) | 4 (20) |
Each tested on 2 to 3 eggs with similar results.
The negative and positive control media were RPMI-1640 medium alone or containing FGF-2 (200 μg/mL), respectively.
The angiogenic response was scored positive when the microvessel area was larger than the mean area plus 3 SD of the negative control medium.22
P < .01 or lower versus nonactive myeloma as a group and subgroups (analysis of variance by Fisher and Kruskal-Wallis test followed by Duncan [t], Bonferroni [t], and Wilcoxon paired tests).