Affected Functions of Cytokine-Stimulated HUVEC Identified by DD/RT-PCR
| Gene Product . | Expression* . | Accession No.† . | Primer Pair‡ . |
|---|---|---|---|
| Leukocyte Trafficking | |||
| MCP-1 (small inducible cytokine A2) | (E) | GB:X14768 | AG3, CT3, GT3 |
| GM-CSF (CSF2) | (L) | GB:M11220 | AG7, GA7 |
| Interleukin-8 | (E) | GB:M28130 | GC3 |
| RANTES (small inducible cytokine A5) | (L) | GB:M21121 | AC6 |
| PCTA-1 | (L) | GB:L78132 | CC12 |
| Apoptosis/Cell Proliferation | |||
| A20: TNF-α–inducible transcriptional factor | (E) | GB:M59465 | AA4, AG4 |
| Novel transcript CA2_1 (hIAP-1, U45878) | (E) | This study | AC2, CA2, GC2 |
| Novel cDNA AG8_1 (stannin) | (E) | This study | AG8 |
| BTG-1 antiproliferative protein | (E) | GB:H70177 | CG11 |
| GSPT1 G to S phase transition | (R) | GB:X17644 | AT7 |
| RGS-5 Regulator of G-protein signaling | (R) | GB:AB008109 | CA2 |
| Protection Against Oxidative Damage | |||
| Manganese superoxide dismutase | (L) | GB:X07834 | GA12 |
| Ferritin heavy-chain | (REG) | GB:M97164 | CC5 |
| Signaling/Regulation of Transcription | |||
| GBP-2 interferon-induced | (L) | GB:M55542 | CC10 |
| SUPT4H | (L) | GB:U43923 | CG11 |
| Fibrillarin | (REG) | GB:X56597 | GA4 |
| TRIP7 | (E) | GB:L40357 | CT8 |
| Cell Shape/Cytoskeleton | |||
| Myosin light chain (alkali isoform) | (E) | GB:M22919 | AA6 |
| GRAVIN | (REG) | GB:AB003476 | GG11 |
| Vesicular Transport | |||
| Annexin-XI | (REG) | GB:L19605 | GG1 |
| RAC1 | (L) | GB:M29870 | GG9 |
| Novel cDNA GG10_2 (Rabkinesin-6) | (R) | This study | GG10 |
| Thrombosis/Hemostasis | |||
| Tissue factor | (E) | GB:M27436 | CACA |
| TFPI-2 (PP5) | (L) | GB:L27624 | GG12 |
| Unassigned | |||
| COX-2 (prostaglandin G/H synthase) | (REG) | GB:D28235 | GG11 |
| Ribosomal protein S11 | (REG) | GB:X06617 | GA2 |
| Novel cDNA GG2_1 | (E) | This study | GG2 |
| Novel cDNA CG12_1 (APOL-like) | (L) | This study | CG12 |
| Gene Product . | Expression* . | Accession No.† . | Primer Pair‡ . |
|---|---|---|---|
| Leukocyte Trafficking | |||
| MCP-1 (small inducible cytokine A2) | (E) | GB:X14768 | AG3, CT3, GT3 |
| GM-CSF (CSF2) | (L) | GB:M11220 | AG7, GA7 |
| Interleukin-8 | (E) | GB:M28130 | GC3 |
| RANTES (small inducible cytokine A5) | (L) | GB:M21121 | AC6 |
| PCTA-1 | (L) | GB:L78132 | CC12 |
| Apoptosis/Cell Proliferation | |||
| A20: TNF-α–inducible transcriptional factor | (E) | GB:M59465 | AA4, AG4 |
| Novel transcript CA2_1 (hIAP-1, U45878) | (E) | This study | AC2, CA2, GC2 |
| Novel cDNA AG8_1 (stannin) | (E) | This study | AG8 |
| BTG-1 antiproliferative protein | (E) | GB:H70177 | CG11 |
| GSPT1 G to S phase transition | (R) | GB:X17644 | AT7 |
| RGS-5 Regulator of G-protein signaling | (R) | GB:AB008109 | CA2 |
| Protection Against Oxidative Damage | |||
| Manganese superoxide dismutase | (L) | GB:X07834 | GA12 |
| Ferritin heavy-chain | (REG) | GB:M97164 | CC5 |
| Signaling/Regulation of Transcription | |||
| GBP-2 interferon-induced | (L) | GB:M55542 | CC10 |
| SUPT4H | (L) | GB:U43923 | CG11 |
| Fibrillarin | (REG) | GB:X56597 | GA4 |
| TRIP7 | (E) | GB:L40357 | CT8 |
| Cell Shape/Cytoskeleton | |||
| Myosin light chain (alkali isoform) | (E) | GB:M22919 | AA6 |
| GRAVIN | (REG) | GB:AB003476 | GG11 |
| Vesicular Transport | |||
| Annexin-XI | (REG) | GB:L19605 | GG1 |
| RAC1 | (L) | GB:M29870 | GG9 |
| Novel cDNA GG10_2 (Rabkinesin-6) | (R) | This study | GG10 |
| Thrombosis/Hemostasis | |||
| Tissue factor | (E) | GB:M27436 | CACA |
| TFPI-2 (PP5) | (L) | GB:L27624 | GG12 |
| Unassigned | |||
| COX-2 (prostaglandin G/H synthase) | (REG) | GB:D28235 | GG11 |
| Ribosomal protein S11 | (REG) | GB:X06617 | GA2 |
| Novel cDNA GG2_1 | (E) | This study | GG2 |
| Novel cDNA CG12_1 (APOL-like) | (L) | This study | CG12 |
Expression kinetics are indicated as follows: E, early genes show peak expression at 1.5 to 3 hours; L, expression of delayed early (3 to 6 hours) and late genes (6 to 20 hours); REG, genes that are expressed relatively abundantly in resting cells, but are upregulated twofold to fourfold; R, repressed genes.
Genbank accession number for obtaining full name, sequence, and references to published reports.
Primer pair represents the combination of anchored primer (letters) and decamer (number) that identified this gene during DD/RT-PCR, as described in Fig 2 and Bauer et al.15