Frequency of Apoptotic Cell Death Among Single Fresh CD34+LIN− Cells, Single Fresh CD34+CD38− Cells, and the Same Cells After 1, 2, or 6 Cell Divisions in Unicellular-Unilineage Erythroid Culture
| . | 34+Lin− (fresh cells) . | 34+38− (fresh cells) . | 34+Lin− 1 Cell Division . | 34+38− 1 Cell Division . | 34+Lin− 2 Cell Divisions . | 34+38− 2 Cell Divisions . | 34+Lin− 6 Cell Divisions . | 34+38− 6 Cell Divisions . |
|---|---|---|---|---|---|---|---|---|
| Experiment no. 1 | 50/25 | 288/276 | 35/1 | 35/1 | 35/1 | 35/1 | 50/2 | 50/1 |
| Experiment no. 2 | 60/45 | 300/296 | 35/1 | 35/1 | 35/1 | 35/1 | 36 | 96/1 |
| Experiment no. 3 | 96/53 | 288/285 | 144/2 | 144/5 | 144/2 | 144/1 | 72/1 | 72/2 |
| Experiment no. 4 | 60/22 | 288/279 | 144/1 | 96/1 | 96/1 | 96/2 | ND | ND |
| % Apoptotic cells (mean ± SD) | 54 ± 16 | 97.5 ± 1.5 | 1.9 ± 1 | 2.5 ± 1 | 2 ± 1 | 2 ± 1 | 1.8 ± 2 | 1.9 ± 1 |
| Range (%) | 37-75 | 96-99 | 0.7-2.8 | 1-3.5 | 1-2.8 | 0.7-2.8 | 0-4 | 1-2.8 |
| . | 34+Lin− (fresh cells) . | 34+38− (fresh cells) . | 34+Lin− 1 Cell Division . | 34+38− 1 Cell Division . | 34+Lin− 2 Cell Divisions . | 34+38− 2 Cell Divisions . | 34+Lin− 6 Cell Divisions . | 34+38− 6 Cell Divisions . |
|---|---|---|---|---|---|---|---|---|
| Experiment no. 1 | 50/25 | 288/276 | 35/1 | 35/1 | 35/1 | 35/1 | 50/2 | 50/1 |
| Experiment no. 2 | 60/45 | 300/296 | 35/1 | 35/1 | 35/1 | 35/1 | 36 | 96/1 |
| Experiment no. 3 | 96/53 | 288/285 | 144/2 | 144/5 | 144/2 | 144/1 | 72/1 | 72/2 |
| Experiment no. 4 | 60/22 | 288/279 | 144/1 | 96/1 | 96/1 | 96/2 | ND | ND |
| % Apoptotic cells (mean ± SD) | 54 ± 16 | 97.5 ± 1.5 | 1.9 ± 1 | 2.5 ± 1 | 2 ± 1 | 2 ± 1 | 1.8 ± 2 | 1.9 ± 1 |
| Range (%) | 37-75 | 96-99 | 0.7-2.8 | 1-3.5 | 1-2.8 | 0.7-2.8 | 0-4 | 1-2.8 |
Values are the number of individually plated cells monitored for apoptosis/number of apoptotic cells.
Abbreviation: ND, not determined.