Myeloid and Erythroid Progenitors in the GPIIb-IIIa+ CD45+ Cell Population From E3.5 to 4 Intraaortic Area
| Culture Conditions . | Colony No./10,000 GPIIb-IIIa +/CD45+ Cells . | |||||
|---|---|---|---|---|---|---|
| Tb/Eb . | Tc . | Ec . | M . | G . | M/G . | |
| Exp I | ||||||
| Myeloid | 47 | 13 | 0 | 513 | 7 | 0 |
| Erythroid | 13 | 20 | 60 | 540 | 40 | 7 |
| Exp II | ||||||
| Myeloid | 27 | 5 | 0 | 275 | 5 | 0 |
| Erythroid | 16 | 38 | 77 | 407 | 0 | 11 |
| Exp III | ||||||
| Myeloid | 94 | 89 | 0 | 122 | 5 | 10 |
| Erythroid | 61 | 10 | 0 | 194 | 55 | 5 |
| Culture Conditions . | Colony No./10,000 GPIIb-IIIa +/CD45+ Cells . | |||||
|---|---|---|---|---|---|---|
| Tb/Eb . | Tc . | Ec . | M . | G . | M/G . | |
| Exp I | ||||||
| Myeloid | 47 | 13 | 0 | 513 | 7 | 0 |
| Erythroid | 13 | 20 | 60 | 540 | 40 | 7 |
| Exp II | ||||||
| Myeloid | 27 | 5 | 0 | 275 | 5 | 0 |
| Erythroid | 16 | 38 | 77 | 407 | 0 | 11 |
| Exp III | ||||||
| Myeloid | 94 | 89 | 0 | 122 | 5 | 10 |
| Erythroid | 61 | 10 | 0 | 194 | 55 | 5 |
Sorted cells from GPIIb-IIIa+ CD45+ cells (population 2 in Fig 6) were cultured in myeloid and erythroid differentiation medium. The number of colonies developed from GPIIb-IIIa+ CD45+ is calculated for 10,000 cells and 3 experiments (Exp) were performed. No cells from populations 1 and 3 differentiated under these conditions.