Percentage of Chimerism After Intrathymic Injection of Bone Marrow–Sorted Cells
| Cell Type . | Sorted Cells . | No. of Cells Injected/ Thymic Lobe . | % of Chimerism . |
|---|---|---|---|
| E14 bone marrow | c-kit+/GPIIb-IIIa− c-kit+/GPIIb-IIIa+ c-kit+/GPIIb-IIIa+ | 1,000 1,000 100 | 3.7 ± 0.8 (7) 20.9 ± 4.3 (10) 5.8 ± 2.0 (5) |
| Adult bone marrow | c-kit+/GPIIb-IIIa− c-kit+/GPIIb-IIIa+lo | 1,000 1,000 | 0.1 (2) 10.0 ± 3.9 (3) |
| Cell Type . | Sorted Cells . | No. of Cells Injected/ Thymic Lobe . | % of Chimerism . |
|---|---|---|---|
| E14 bone marrow | c-kit+/GPIIb-IIIa− c-kit+/GPIIb-IIIa+ c-kit+/GPIIb-IIIa+ | 1,000 1,000 100 | 3.7 ± 0.8 (7) 20.9 ± 4.3 (10) 5.8 ± 2.0 (5) |
| Adult bone marrow | c-kit+/GPIIb-IIIa− c-kit+/GPIIb-IIIa+lo | 1,000 1,000 | 0.1 (2) 10.0 ± 3.9 (3) |
Sorted cells from H.B19 ov+ chickens were injected into the thymus of 8-day-old irradiated congenic H.B19 ov− chickens. The chimerism of the recipient’s thymus was determined 2 weeks later by flow cytometry with the 11A9 MoAb, which recognizes the ov+ antigen. Numbers in parentheses indicate the number of animals used for each condition.