Ratios of c-mpl mRNA to GpIIb mRNA Levels in PLTs From Normal and TPO-Stimulated Mice and in MKs in the Presence and Absence of TPO
| . | PLTs* . | ||
|---|---|---|---|
| Control Mice . | TPO-Stimulated Mice (model 1) . | TPO-Stimulated Mice (model 2) . | |
| 0.26 ± 0.02 | 0.28 ± 0.01 | 0.25 ± 0.01 | |
| . | PLTs* . | ||
|---|---|---|---|
| Control Mice . | TPO-Stimulated Mice (model 1) . | TPO-Stimulated Mice (model 2) . | |
| 0.26 ± 0.02 | 0.28 ± 0.01 | 0.25 ± 0.01 | |
| . | MKs (condition no. 1)† . | |
|---|---|---|
| SCF, IL-6, IL-3 . | TPO . | |
| 0.21 ± 0.02 | 0.20 ± 0.02 | |
| MKs (condition no. 2)‡ | ||
| SCF, IL-6, IL-3 | TPO | |
| 0.19 ± 0.01 | 0.18 ± 0.005 | |
| . | MKs (condition no. 1)† . | |
|---|---|---|
| SCF, IL-6, IL-3 . | TPO . | |
| 0.21 ± 0.02 | 0.20 ± 0.02 | |
| MKs (condition no. 2)‡ | ||
| SCF, IL-6, IL-3 | TPO | |
| 0.19 ± 0.01 | 0.18 ± 0.005 | |
The ratios were calculated from the quantification of protected fragments performed with a phosphorimager system. Each indicated ratio represents the mean (±SD) of ratios obtained with the independent preparations.
PLTs were isolated from control mice, from mice grafted subcutaneously with FDC-P1 cells producing TPO (model 1) or from mice in which the hematopoietic cells produce TPO (model 2).
MKs obtained in the presence of TPO (3 days) were either maintained in TPO or shifted for 24 additional hours to a medium deprived of TPO, but containing a combination of SCF, IL-6, and IL-3.
MKs were obtained either in the presence of TPO or in the presence of a combination of SCF, IL-6, and IL-3 (4 days).