Effects of Human SCF on Colony Formation of Marmoset Bone Marrow Progenitor Cells
| Cytokine* . | CFU-GM . | BFU-E . | CFU-Mix . | Cytokine† . | CFU-MK . |
|---|---|---|---|---|---|
| Epo | 3.5 ± 1.4 | 18.6 ± 2.4 | 0.0 ± 0.0 | IL-3 | 8.6 ± 1.5 |
| IL-3 | 68.0 ± 7.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | Tpo | 22.9 ± 2.2 |
| SCF + Epo | 112.5 ± 6.0 | 51.0 ± 3.1 | 6.0 ± 1.0 | SCF + Tpo | 36.9 ± 2.8 |
| SCF + IL-3 | 135.5 ± 4.8 | 1.0 ± 0.6 | 4.8 ± 0.6 | SCF + IL-3 + Tpo | 47.9 ± 3.2 |
| SCF + IL-3 + Epo | 105.5 ± 3.3 | 67.5 ± 4.1 | 24.0 ± 1.6 | ||
| GM-CSF | 61.5 ± 1.9 | 0.0 ± 0.0 | 0.0 ± 0.0 | ||
| SCF + GM-CSF | 134.5 ± 2.9 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| Cytokine* . | CFU-GM . | BFU-E . | CFU-Mix . | Cytokine† . | CFU-MK . |
|---|---|---|---|---|---|
| Epo | 3.5 ± 1.4 | 18.6 ± 2.4 | 0.0 ± 0.0 | IL-3 | 8.6 ± 1.5 |
| IL-3 | 68.0 ± 7.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | Tpo | 22.9 ± 2.2 |
| SCF + Epo | 112.5 ± 6.0 | 51.0 ± 3.1 | 6.0 ± 1.0 | SCF + Tpo | 36.9 ± 2.8 |
| SCF + IL-3 | 135.5 ± 4.8 | 1.0 ± 0.6 | 4.8 ± 0.6 | SCF + IL-3 + Tpo | 47.9 ± 3.2 |
| SCF + IL-3 + Epo | 105.5 ± 3.3 | 67.5 ± 4.1 | 24.0 ± 1.6 | ||
| GM-CSF | 61.5 ± 1.9 | 0.0 ± 0.0 | 0.0 ± 0.0 | ||
| SCF + GM-CSF | 134.5 ± 2.9 | 0.0 ± 0.0 | 0.0 ± 0.0 |
Data are expressed as the mean ± SEM from three separate experiments performed in triplicate. Human SCF: 10 ng/mL, IL-3: 10 ng/mL, Epo: 2 U/mL, GM-CSF: 10 ng/mL, Tpo: 10 ng/mL.
Progenitor cell assays were performed in serum-free conditions. CFU-GM, BFU-E, CFU-Mix/2 × 104 marmoset bone marrow MNCs.
Progenitor cell assays were performed using human platelet poor plasma. CFU-MK/1 × 104 marmoset bone marrow MNCs.