The Types and the Number of Colonies That Could Be Morphologically Identified at 4 to 8 Weeks in Culture (See Fig 1)
| Types of Colonies . | No. of Colonies . |
|---|---|
| Single | |
| Mast | 13 |
| (plate A only)4-150 | (8) |
| (plate A and B)4-151 | (5) |
| Ba (plate B only) | 12 |
| Eo (plate B only) | 4 |
| M | 26 |
| (plate A only) | (1) |
| (plate B only) | (12) |
| (plate A and B) | (13) |
| Double | |
| Mast + Ba (plate A and B)‡ | 1 |
| Ba + Eo (plate B only) | 10 |
| Ba + M (plate B only) | 1 |
| Eo + M | 6 |
| (plate B only) | (2) |
| (plate A and B) | (4) |
| Triple | |
| Mast + Ba + Eo (plate B only) | 1 |
| Ba + Eo + M (plate B only) | 7 |
| Multiple | |
| Mast + Ba + Eo + M (plate A and B) | 7 |
| Types of Colonies . | No. of Colonies . |
|---|---|
| Single | |
| Mast | 13 |
| (plate A only)4-150 | (8) |
| (plate A and B)4-151 | (5) |
| Ba (plate B only) | 12 |
| Eo (plate B only) | 4 |
| M | 26 |
| (plate A only) | (1) |
| (plate B only) | (12) |
| (plate A and B) | (13) |
| Double | |
| Mast + Ba (plate A and B)‡ | 1 |
| Ba + Eo (plate B only) | 10 |
| Ba + M (plate B only) | 1 |
| Eo + M | 6 |
| (plate B only) | (2) |
| (plate A and B) | (4) |
| Triple | |
| Mast + Ba + Eo (plate B only) | 1 |
| Ba + Eo + M (plate B only) | 7 |
| Multiple | |
| Mast + Ba + Eo + M (plate A and B) | 7 |
The clone-sorted cells were divided into two parts after 1 week and were cultured either with SF + IL-6 (plate A in Fig 1) or IL-3 alone (plate B in Fig 1). Other than the four cell types (Mast, mast cells; Ba, basophils; Eo, eosinophils; M, macrophages) shown here, a few immature cells were sometimes found in the colonies, although the colonies consisting of largely mast cells with a few immature cells were evaluated as mast cell colonies.
Mast cells were found only in plate A but not in plate B.
Mast cells were found both in plate A and B.
Mast cells were found in plate A and mast cells, basophils, and blastoid immature cells were found in plate B.