Table 2. SF/IL-6–Dependent Pure Mast... | American Society of Hematology

Table 2.

SF/IL-6–Dependent Pure Mast Cell Colony Formation Derived From Single CD34⁺ Cells

Phenotype	Plated Single Cells	Total Colonies	Pure Mast Cell Colonies
CD34⁺CD38⁺	768	649	34
CD34⁺CD38⁻	576	334	0

CD34⁺HLA-DR⁺	194	173	1
CD34⁺HLA-DR⁻	189	159	18

CD34⁺CD33⁺	192	154	9
CD34⁺CD33⁻	192	130	5

Phenotype	Plated Single Cells	Total Colonies	Pure Mast Cell Colonies
CD34⁺CD38⁺	768	649	34
CD34⁺CD38⁻	576	334	0

CD34⁺HLA-DR⁺	194	173	1
CD34⁺HLA-DR⁻	189	159	18

CD34⁺CD33⁺	192	154	9
CD34⁺CD33⁻	192	130	5

Cord blood cells clone-sorted by FACS-Vantage (Becton Dickinson) equipped with an ACDU were cultured in the presence of SF and IL-6 for 8 weeks and were stained with antitryptase MoAb. Other than the pure mast cell colonies shown here, 10 of 200 mixed colonies were found to have both tryptase-positive mast cells, tryptase-negative large cells (macrophages), and undifferentiated cells.

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