Table 1. Phenotypic Analysis of Cord... | American Society of Hematology

Table 1.

Phenotypic Analysis of Cord Blood-Derived Hematopoietic Progenitors

Phenotype	G	GM	M	Eo	E	Mix	Bl	Total
CD34⁺CD38⁺	15	55	105	8	27	35	2	258
CD34⁺CD38⁻	8	64	76	0	2	34	94	278
CD34⁺HLA-DR⁺	4	43	30	4	22	35	3	147
CD34⁺HLA-DR⁻	9	51	47	7	8	24	3	161
CD34⁺CD33⁺	16	60	40	8	18	11	10	167
CD34⁺CD33⁻	7	18	19	3	22	50	16	140

Phenotype	G	GM	M	Eo	E	Mix	Bl	Total
CD34⁺CD38⁺	15	55	105	8	27	35	2	258
CD34⁺CD38⁻	8	64	76	0	2	34	94	278
CD34⁺HLA-DR⁺	4	43	30	4	22	35	3	147
CD34⁺HLA-DR⁻	9	51	47	7	8	24	3	161
CD34⁺CD33⁺	16	60	40	8	18	11	10	167
CD34⁺CD33⁻	7	18	19	3	22	50	16	140

Cord blood cells clone-sorted by FACS-Vantage (Becton Dickinson) equipped with an ACDU (Becton Dickinson) were cultured in 200 μL methylcellulose medium in 288 wells (CD34⁺CD38⁺or CD34⁺CD38⁻) or 192 wells (CD34⁺HLA-DR⁺ or CD34⁺HLA-DR⁻, CD34⁺CD33⁺, or CD34⁺CD33⁻) and were scored at day 14 according to criteria previously reported.34 35 To assess the accuracy of the in situ identification of colonies, individual colonies were morphologically examined with May-Grünwald-Giemsa staining.

Abbreviations: G, granulocyte (neutrophil) colonies; M, macrophage colonies; GM, granulocyte (neutrophil)-macrophage colonies; E, erythroid bursts; Eo, eosinophil colonies; Mix, mixed colonies; BL, blast cell colonies.

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