Table 3. Pertussis Toxin Treatment... | American Society of Hematology

Table 3.

Pertussis Toxin Treatment Blocks IL-8–Induced Migration of Mast Cells

Chemokine	PTx (ng/mL)	Cells/3HPF (±SEM)
IL-8	—	108 (6)
	100	43 (5)^3-150
	1,000	26 (5)^3-151
PF-4	—	19 (6)
	100	26 (4)
	1,000	12 (7)
SCF	—	187 (14)
	100	174 (9)
	1,000	178 (9)

Chemokine	PTx (ng/mL)	Cells/3HPF (±SEM)
IL-8	—	108 (6)
	100	43 (5)^3-150
	1,000	26 (5)^3-151
PF-4	—	19 (6)
	100	26 (4)
	1,000	12 (7)
SCF	—	187 (14)
	100	174 (9)
	1,000	178 (9)

HMC-1 cells were incubated for 90 minutes at 37°C before the chemotaxis experiment with either 0, 100, or 1,000 ng/mL pertussis toxin. The treated cells were tested for their ability to respond to IL-8 (100 ng/mL), PF-4 (100 ng/mL), or SCF (100 ng/mL).

F3-150

P < .05.

F3-151

P < .01 (n = 3).

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